Polyfunctional CD4 T-cells correlate with in vitro mycobacterial growth inhibition following Mycobacterium bovis BCG-vaccination of infants

• Published in: Vaccine Vol. 34; no. 44; pp. 5298 - 5305
• Main Authors: Smith, Steven G, Zelmer, Andrea, Blitz, Rose, Fletcher, Helen A, Dockrell, Hazel M
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Ltd 17.10.2016; Elsevier Limited
• Subjects: Allergy and Immunology; Antigens; BCG; BCG Vaccine - administration & dosage; BCG Vaccine - immunology; CD4-Positive T-Lymphocytes - immunology; Cytokines; Cytokines - biosynthesis; Cytokines - immunology; Female; Flow Cytometry; HIV; Human immunodeficiency virus; Humans; Hypotheses; Infant; Infants; Interferon-gamma - biosynthesis; Interferon-gamma - genetics; Interleukin-2 - biosynthesis; Interleukin-2 - genetics; Leukocytes, Mononuclear - immunology; Leukocytes, Mononuclear - microbiology; Lymphocyte Activation; Male; Mycobacterial growth inhibition; Mycobacterium bovis; Mycobacterium bovis - growth & development; Mycobacterium bovis - immunology; Mycobacterium tuberculosis; Polyfunctional; T-cells; Th17 Cells - immunology; Tuberculin - immunology; Tuberculosis; Vaccines; United Kingdom > UK; T-cells; Staphylococcus enterotoxin B; Cytokines; Polyfunctional; PPD; purified protein derivative; NTM; BCG; tuberculosis; Infants; IQR; interquartile range; TB; Mycobacterial growth inhibition; intracellular cytokine staining; Bacillus Calmette-Guerin; SEB; MGIA; ICS; mycobacterial growth inhibition assay; non-tuberculous mycobacteria
• ISSN: 0264-410X; 1873-2518
• DOI: 10.1016/j.vaccine.2016.09.002

Highlights • BCG vaccination induces predominantly polyfunctional CD4 T-cells in infants. • BCG vaccination induces Th-17 response in infants. • Capacity of PBMC to inhibit mycobacterial growth in vitro enhanced after BCG. • Correlation between polyfunctional T-cells and growth inhibition after BCG.