Generation of a pig induced pluripotent stem cell (piPSC) line from embryonic fibroblasts by incorporating LIN28 to the four transcriptional factor-mediated reprogramming: VSMUi001-D

• Published in: Stem cell research Vol. 24; no. C; pp. 21 - 24
• Main Authors: Chakritbudsabong, Warunya, Sariya, Ladawan, Pamonsupornvichit, Sirikron, Pronarkngver, Rassmeepen, Chaiwattanarungruengpaisan, Somjit, Ferreira, Joao N., Setthawong, Piyathip, Phakdeedindan, Praopilas, Techakumphu, Mongkol, Tharasanit, Theerawat, Rungarunlert, Sasitorn
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 01.10.2017; Elsevier
• Subjects: Animals; Cell Differentiation; Cell Line; Cellular Reprogramming - genetics; Fibroblasts - metabolism; Humans; Induced Pluripotent Stem Cells - cytology; Induced Pluripotent Stem Cells - metabolism; RNA-Binding Proteins - genetics; RNA-Binding Proteins - metabolism; Swine; Transcription Factors - metabolism; Transfection
• ISSN: 1873-5061; 1876-7753
• DOI: 10.1016/j.scr.2017.08.005

Pig induced pluripotent stem cell (piPSC) line was generated from embryonic fibroblast cells using retroviral transduction approaches carrying human transcriptional factors: OCT4, SOX2, KLF4, c-MYC and LIN28. The generated piPSC line, VSMUi001-D, was positive for alkaline phosphatase activity and expressed the pluripotency associated transcription factors including OCT4, SOX2, NANOG and surface markers SSEA-1, all iPSC hallmarks of authenticity. Furthermore, VSMUi001-D exhibited a normal karyotype and formed embryoid bodies in vitro and teratomas in vivo. Upon cardiac differentiation, VSMUi001-D displayed spontaneous beating and expressed cardiomyocyte markers, like cardiac Troponin T.