Toxicity monitoring of solvents, hydrocarbons, and heavy metals using statistically optimized model of luminous Vibrio sp. 6HFE
Background The utilization of bioluminescent bacteria in environmental monitoring of water contaminates considers being a vital and powerful approach. This study aimed to isolate, optimize, and apply luminescent bacteria for toxicity monitoring of various toxicants in wastewater. Results On the basi...
Saved in:
Published in | Journal of Genetic Engineering and Biotechnology Vol. 20; no. 1; pp. 91 - 17 |
---|---|
Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
Berlin/Heidelberg
Springer Berlin Heidelberg
01.07.2022
Springer Elsevier |
Subjects | |
Online Access | Get full text |
Cover
Loading…
Summary: | Background
The utilization of bioluminescent bacteria in environmental monitoring of water contaminates considers being a vital and powerful approach. This study aimed to isolate, optimize, and apply luminescent bacteria for toxicity monitoring of various toxicants in wastewater.
Results
On the basis of light intensity, strain
Vibrio
sp. 6HFE was initially selected, physiologically/morphologically characterized, and identified using the 16SrDNA gene. The luminescence production was further optimized by employing statistical approaches (Plackett-Burman design and central composite design). The maximum bioluminescence intensity recorded 1.53 × 10
6
CPS using optimized medium containing (g/L), yeast extract (0.2g), CaCl
2
(4.0), MgSO
4
(0.1), and K
2
HPO
4
(0.1) by 2.3-fold increase within 1h. The harnessing of
Vibrio
sp. 6HFE as a bioluminescent reporter for toxicity of organic solvents was examined using a bioluminescence inhibition assay. According to IC
50
results, the toxicity order of such pollutants was chloroform > isoamyl > acetic acid > formamide > ethyl acetate > acetonitrile > DMSO > acetone > methanol. However, among eight heavy metals tested, the bioluminescence was most sensitive to Ag
+
and Hg
+
and least sensitive to Co
2+
and Ni
2+
. Additionally, the bioluminescence was inhibited by benzene, catechol, phenol, and penta-chlorophenol at 443.1, 500, 535.1, and 537.4 ppm.
Conclusion
Vibrio
sp. 6HFE succeeded in pollution detection at four different environmental and wastewater samples revealing its efficiency in ecotoxicity monitoring. |
---|---|
ISSN: | 1687-157X 2090-5920 |
DOI: | 10.1186/s43141-022-00360-1 |