Transcriptomic analysis of the host response to an iridovirus infection in Chinese giant salamander, Andrias davidianus

• Published in: Veterinary research (Paris) Vol. 46; no. 136; p. 136
• Main Authors: Fan, Yuding, Chang, Ming Xian, Ma, Jie, LaPatra, Scott E, Hu, Yi Wei, Huang, Lili, Nie, Pin, Zeng, Lingbing
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 20.11.2015; BioMed Central
• Subjects: Animals; DNA Virus Infections - genetics; DNA Virus Infections - veterinary; DNA Virus Infections - virology; DNA viruses; Expressed Sequence Tags; Genetic aspects; Genetic transcription; Host-virus relationships; Life Sciences; Medical research; Observations; Ranavirus - physiology; Spleen - metabolism; Spleen - virology; Transcriptome; Urodela - genetics; Veterinary medicine
• ISSN: 1297-9716; 0928-4249; 1297-9716
• DOI: 10.1186/s13567-015-0279-8

The emergence of an infectious viral disease caused by the Chinese giant salamander iridovirus (GSIV) has led to substantial economic losses. However, no more molecular information is available for the understanding of the mechanisms associated with virus-host interaction. In this study, de novo sequencing was used to obtain abundant high-quality ESTs and investigate differentially-expressed genes in the spleen of Chinese giant salamanders that were either infected or mock infected with GSIV. Comparative expression analysis indicated that 293 genes were down-regulated and 220 genes were up-regulated. Further enrichment analysis showed that the most enriched pathway is "complement and coagulation cascades", and significantly enriched diseases include "inherited thrombophilia", "immune system diseases", "primary immunodeficiency", "complement regulatory protein defects", and "disorders of nucleotide excision repair". Additionally, 30 678 simple sequence repeats (SSRs) from all spleen samples, 26 355 single nucleotide polymorphisms (SNPs) from the spleens of uninfected animals and 36 070 SNPs from the spleens of infected animals were detected. The large amount of variation was specific for the Chinese giant salamanders that were infected with GSIV. The results reported herein provided significant and new EST information that could contribute greatly in investigations into the molecular functions of immune genes in the Chinese giant salamander.