Expression and subcellular localization of E-cadherin, alpha-catenin, and beta-catenin in 8 feline mammary tumor cell lines

• Published in: Journal of Veterinary Medical Science Vol. 69; no. 8; pp. 831 - 834
• Main Authors: Takauji, S.(Tokyo Univ. (Japan)), Watanabe, M, Uyama, R, Nakagawa, T, Miyajima, N, Mochizuki, M, Nishimura, R, Sugano, S, Sasaki, N
• Format: Journal Article
• Language: English
• Published: Japan JAPANESE SOCIETY OF VETERINARY SCIENCE 01.08.2007
• Subjects: alpha Catenin - genetics; alpha Catenin - metabolism; Animals; beta Catenin - genetics; beta Catenin - metabolism; Cadherins - genetics; Cadherins - metabolism; CATS; CELL CULTURE; Cell Line, Tumor; CHAT; CULTIVO DE CELULAS; CULTURE DE CELLULE; E-cadherin; EVOLUTION MALIGNE; feline mammary tumor; GATO; Gene Expression Regulation, Neoplastic; GLANDE MAMMAIRE; GLANDULAS MAMARIAS; IMMUNOBLOTTING; IMMUNOFLUORESCENCE; INMUNOFLUORESCENCIA; MALIGNANT COURSE; MAMMARY GLANDS; Mammary Neoplasms, Animal - metabolism; Neoplasm Proteins - genetics; Neoplasm Proteins - metabolism; NEOPLASMAS; NEOPLASME; NEOPLASMS; PROCESO MALIGNO; PROTEINAS; PROTEINE; PROTEINS; α- and β-catenin
• ISSN: 0916-7250; 1347-7439
• DOI: 10.1292/jvms.69.831

Protein expression and subcellular localization of E-cadherin, alpha-catenin, and beta-catenin in 8 feline mammary tumor cell lines were examined by western blot analysis and fluorescence immunocytochemistry. A low E-cadherin expression was observed in FNN-m cells. Furthermore, compared to other cell lines, two E-cadherin bands existed in, FMC-p1 cells and were localized in the perinuclear region; distinct radial lines were observed in the cytoplasm. A low alpha-catenin expression was observed in FON-m cells, but there were no apparent abnormalities in its localization. In contrast, similar levels of beta-catenin expression and cytoplasmic localization were observed in all cell lines.