Changes of gonadotropin-releasing hormone receptor 2 during the anadromous spawning migration in Coilia nasus

• Published in: BMC developmental biology Vol. 16; no. 1; p. 42
• Main Authors: Duan, Jin-Rong, Fang, Di-An, Zhang, Min-Ying, Liu, Kai, Zhou, Yan-Feng, Xu, Dong-Po, Xu, Pao, Li, Da-Peng
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 24.11.2016; BioMed Central
• Subjects: Analysis; Anchovies; Animal Migration; Animals; Brain - metabolism; Female; Fish Proteins - genetics; Fish Proteins - metabolism; Fishes - embryology; Fishes - physiology; Gonadotropin releasing hormone; Gonadotropin-Releasing Hormone - genetics; Gonadotropin-Releasing Hormone - metabolism; Ovary - metabolism; Signal Transduction; Spawning; Coilia nasus; Spawning upstream migration; GnRH-R2; Yangtze River
• ISSN: 1471-213X; 1471-213X
• DOI: 10.1186/s12861-016-0142-9

An increase in the activity of the pituitary-gonad axis (PG-axis) and gonad development are essential for the onset of spawning migration in teleosts. In the fish Coilia nasus, gonad development and spawning migration up the Yangtze River occurs by the end of each summer. We hypothesized that gonadotropin releasing hormones receptor 2 (GnRH-R2), which together produce a signal that interacts with the PG-axis, may help to regulate spawning migration processes. In this regard, we (1) characterized the gonadosomatic index (GSI) in the anadromous fish C. nasus; (2) analyzed the GnRH-R2 mRNA expression levels in ovary and brain, and concentrations in the serum; and (3) identified the GnRH-R2 protein distribution in the brain and ovaries. We found strong relationships between all of these indices. The results indicate that GnRH-R2 could act together to promote spawning during the anadromous migration. There is some evidence that the GnRH-R2 gene expression levels and protein distributions change in association with the migratory behavior.