ANTIVIRAL EFFECT OF OXETANOCIN G AGAINST GUINEA PIG CYTOMEGALOVIRUS INFECTION IN VITRO AND IN VIVO

• Published in: Journal of antibiotics Vol. 43; no. 12; pp. 1573 - 1578
• Main Authors: YAMAMOTO, NAOHIKO, YAMADA, YOSHINARI, DAIKOKU, TOHRU, NISHIYAMA, YUKIHIRO, TSUTSUI, YOSHIHIRO, SHIMADA, NOBUYOSHI, TAKAHASHI, KATSUTOSHI
• Format: Journal Article
• Language: English
• Published: Tokyo JAPAN ANTIBIOTICS RESEARCH ASSOCIATION 1990; Japan Antibiotics Research Association
• Subjects: Acyclovir - pharmacology; Animals; Antibiotics. Antiinfectious agents. Antiparasitic agents; Antiviral agents; Antiviral Agents - pharmacology; Antiviral Agents - therapeutic use; Biological and medical sciences; Cell Line; Cells, Cultured; Cytomegalovirus - drug effects; Cytomegalovirus Infections - drug therapy; Fibroblasts; Ganciclovir - pharmacology; Guanine - analogs & derivatives; Guanine - pharmacology; Guanine - therapeutic use; Guinea Pigs; Humans; Medical sciences; Neutralization Tests; Pharmacology. Drug treatments; Salivary Glands - microbiology; Cytomegalovirus; Herpesviridae; Guanosine; Rodentia; Betaherpesvirinae; In vitro; Infection; Virus; In vivo; Vertebrata; Chemotherapy; Experimental disease; Mammalia; Guinea pig; Sensitivity resistance; Treatment; Analog; Animal; Viral disease; Replication
• ISSN: 0021-8820; 1881-1469
• DOI: 10.7164/antibiotics.43.1573

The antiviral activity of a new guanosine analog, 9-(2-deoxy-2-hydroxymethyl-βD-erythro-oxetanosyl)guanine (OXT-G), against guinea pig cytomegalovirus (GPCMV) was evaluated both in vitro and in vivo. In the plaque reduction assay, the median effective concentration (EC50) of OXT-G, ganciclovir (DHPG) and acyclovir (ACV) against GPCMV was 0.03, 6.4 and 52 μg/ml, respectively. The selectivity index, based on the ratio of the median inhibitory concentration for cell growth of guinea pig embryo fibroblasts to the median effective concentration for GPCMV plaque formation, was about 100-fold higer than that of DHPG. In an in vivo study, Hartley guinea pigs infected with GPCMV were treated with OXT-G or DHPG (20 mg/kg/day) for 2 weeks, and it was found that virus titers in the salivary gland were 70-fold lower in OXT-G-treated guinea pigs than in DHPG-treated animals. The results indicate that OXT-G was more potent and selective against GPCMV infection than DHPG.