Co-electrospun fibrous scaffold-adsorbed DNA for substrate-mediated gene delivery

• Published in: Journal of biomedical materials research. Part A Vol. 96A; no. 1; pp. 212 - 220
• Main Authors: Zhang, Jun, Duan, Yajun, Wei, Di, Wang, Lianyong, Wang, Hongjun, Gu, Zhongwei, Kong, Deling
• Format: Journal Article
• Language: English
• Published: Hoboken Wiley Subscription Services, Inc., A Wiley Company 01.01.2011; Wiley-Blackwell
• Subjects: Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy; Animals; Applied cell therapy and gene therapy; Biocompatible Materials - chemistry; Biological and medical sciences; Biotechnology; Cells, Cultured; DNA - chemistry; DNA - metabolism; Electrochemical Techniques - methods; electrospun fibers; Fundamental and applied biological sciences. Psychology; gene delivery; Gene Transfer Techniques; Health. Pharmaceutical industry; HEK293 Cells; Humans; Industrial applications and implications. Economical aspects; Materials Testing; Medical sciences; Mesenchymal Stromal Cells - cytology; Mesenchymal Stromal Cells - physiology; Miscellaneous; multifunctional scaffold; Nanofibers - chemistry; Polyethylene Glycols - chemistry; Polyethyleneimine - chemistry; polyethylenimine (PEI); Rats; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases; Technology. Biomaterials. Equipments; tissue engineering; Transfection; Transfusions. Complications. Transfusion reactions. Cell and gene therapy; Tissue engineering; Electrospinning; Scaffold; electrospun fibers; Genetic transfer; Polyelectrolyte; gene delivery; Treatment; DNA; polyethylenimine (PEI); multifunctional scaffold; Olefinimine polymer; Biomaterial; Gene therapy; Nucleic acid; Polyethylene imine; Biomedical engineering
• ISSN: 1549-3296; 1552-4965; 1552-4965
• DOI: 10.1002/jbm.a.32962

Incorporation of gene into electrospun nanofibers for localized gene transfection of target cells represents a robust platform for tissue regeneration. In this study, a new two‐step approach was explored to immobilize DNA onto electrospun nanofibers for effective gene delivery, that is, nonviral gene vector of polyethylene glycol (PEG)‐modified polyethylenimine (PEI) was incorporated into scaffolds by electrospinning and then target DNA was adsorbed onto the electrospun nanofibers via electrostatic interaction between DNA and PEI–PEG. PEI–PEG/DNA particles formed from the released DNA, and PEI–PEG had a uniform particle size of approximately 200 nm. This nanofiber‐based gene delivery system exhibited high transfection efficiency, in which >65% of human embryonic kidney 293 cells and >40% of mesenchymal stem cells were transfected with green fluorescent protein gene. Compared with PEI, PEG modification of PEI had improved the biocompatibility and further increased the transfection efficiency. These results suggest that the combination of nonviral gene carrier with electrospun nanofibers could be used for localized gene delivery, which has multifold potential applications in tissue engineering or as an in vivo substrate for tissue regeneration. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2010.