Growth behavior of cochlear nucleus neuronal cells on semiconductor substrates

• Published in: Journal of biomedical materials research. Part A Vol. 97A; no. 2; pp. 158 - 166
• Main Authors: Rak, Kristen, Wasielewski, Natalia, Radeloff, Andreas, Scherzed, Agmal, Jablonka, Sibylle, Hagen, Rudolf, Mlynski, Robert
• Format: Journal Article
• Language: English
• Published: Hoboken Wiley Subscription Services, Inc., A Wiley Company 01.05.2011; Wiley-Blackwell
• Subjects: Animals; Auditory Brain Stem Implants; auditory brainstem; Axons - metabolism; Biocompatible Materials - chemistry; Biological and medical sciences; cell differentiation; Cochlear Nucleus - metabolism; Cochlear Nucleus - physiology; Culture Media; Electrodes; Immunohistochemistry - methods; Medical sciences; Microscopy, Electron, Scanning - methods; neuroimaging; Neurons - metabolism; Rats; Rats, Sprague-Dawley; scanning electron microscopy; Semiconductors; Silicon - chemistry; Surface Properties; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases; Technology. Biomaterials. Equipments; Scanning electron microscopy; Semiconductor materials; auditory brainstem; Growth; Brain stem; Central nervous system; Cochlear nucleus; Nervous system; Cell differentiation; semiconductors; Encephalon; neuroimaging; Neuron; Auditory pathway; Biomaterial; Behavior; Biomedical engineering
• ISSN: 1549-3296; 1552-4965; 1552-4965
• DOI: 10.1002/jbm.a.33042

Auditory brainstem implants provide sound information by direct stimulation of the cochlear nucleus to patients with dysfunctional or absent cranial nerve VIII. In contrast to patients with cochlear implants, the use of the auditory brainstem implants is less successful. This cannot be fully explained by the difference location of stimulation but a rather unspecific neuronal stimulation. The aim of this study was to further examine neuronal cells of the cochlear nucleus and to test their interactions with semiconductor substrates as a potential electrode material for improved auditory brainstem implants. The cochlear nuclei of postnatal day 7 rats were microsurgically dissected. The tissue was dissociated enzymatically and plated on coverslips as control and on the semiconductor substrates silicon or silicon nitride. After 4 days in culture the morphology and growth of dissociated cells was determined by fluorescence and scanning electron microscopy. Dissociated cells of the cochlear nucleus showed reduced cell growth on semiconductor substrates compared with controls. SEM analysis demonstrated close contact of neurons with supporting cells in culture and good adherence of neuronal growth cones on the used materials. These findings present basic knowledge for the development of neuron–electrode interfaces for future auditory brainstem implants. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A:, 2011.