M. tuberculosis infection and antigen specific cytokine response in healthcare workers frequently exposed to tuberculosis

• Published in: Scientific reports Vol. 9; no. 1; p. 8201
• Main Authors: Essone, Paulin N., Leboueny, Marielle, Maloupazoa Siawaya, Anicet Christel, Alame-Emane, Amel Kévin, Aboumegone Biyogo, Oriane Cordelia, Dapnet Tadatsin, Patrice Hemery, Mveang Nzoghe, Amandine, Essamazokou, Dimitri Ulrich, Mvoundza Ndjindji, Ofilia, Padzys, Guy-Stéphane, Agnandji, Selidji Todagbe, Takiff, Howard, Gicquel, Brigitte, Djoba Siawaya, Joel Fleury
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 03.06.2019; Nature Publishing Group
• Subjects: 13/21; 631/250/255/1856; 692/308/53; 96/21; Adult; Anthropometry; Antigens; Antigens - immunology; Antigens, Bacterial - immunology; Cross-Sectional Studies; Cytokines; Cytokines - immunology; Female; Granulocyte-macrophage colony-stimulating factor; Health care; Health Personnel; Humanities and Social Sciences; Humans; IL-1β; Immune response; Immunity, Innate; Infections; Inflammation; Interleukin 10; Interleukin 12; Interleukin 2; Interleukin 4; Interleukin 5; Interleukin 6; Interleukin 8; Latent Tuberculosis - immunology; Lymphocytes T; Male; Medical personnel; Middle Aged; multidisciplinary; Mycobacterium tuberculosis; Occupational Diseases - immunology; Occupational Diseases - microbiology; Occupational Exposure; Odds Ratio; Science; Science (multidisciplinary); Tuberculosis; Tuberculosis - immunology; Tumor necrosis factor-α; γ-Interferon
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-019-44294-0

Tuberculosis (TB) is the leading cause of death due to an infectious agent, but only a small fraction of those infected develop the disease. Cytokines are involved in the mediation and regulation of immunity, and their secretion patterns may reflect the infection status. To increase our understanding of immune response to M . tuberculosis infection, we conducted a cross-sectional study investigating M . tuberculosis infection status and comparing the release profiles of cytokines GM-CSF, IFN-γ, IL-1β, IL-10, IL-12 (p70), IL-2, IL-4, IL-5, IL-6, IL-8, TNF-α, in community controls (CCs) and healthy healthcare workers (HCWs) highly exposed to TB. Among HCWs and CCs, the probability of latent M . tuberculosis (LTB + ) infection was respectively 5.4 (p = 0.002) and 3.4 (p = 0.006) times higher in men than women. The odds ratio of LTB infection was 4 times higher among HCWs in direct contact with active TB patients than other HCW (p = 0.01). Whole blood supernatant cytokine responses to M . tuberculosis antigens showed differential pro-inflammatory responses between HCWs and CCs. CCs LTB− had higher IL-1β responses than HCWs LTB− (p = 0.002). HCWs LTB+ had significantly higher IL-8 responses to M . tuberculosis antigens than HCWs LTB− (p = 0.003) and CCs LTB− (p = 0.015). HCWs LTB+/− showed weak but positive TNF-α responses to M . tuberculosis antigen stimulation compared to CCs LTB+/− (p ≤ 0.015). Looking at T-helper (1 and 2) responses, HCWs LTB+ and CCs LTB+ had significantly higher IFN-γ and IL-2 responses compared to HCWs LTB− and CCs LTB− (p < [0.0001–0.003]). Also, TB antigen induced IL-5 secretion was significantly higher in HCWs LTB+ and CCs LTB+ than in non-infected CCs LTB− (p < [0.005–0.04]). M . tuberculosis antigen specific responses in HCWs LTB+ varied based on active TB exposure gradient. HCWs LTB+ who were highly exposed to active TB (≥3 hours per day) had significantly higher IFN-γ and IL-8 responses (p ≤ 0.02) than HCWs LTB+ not in direct contact with active TB patients. HCWs LTB+ working with active TB patients for 5 to 31 years had a significantly enhanced secretion of proinflammatory cytokines (GM-CSF, IFN-γ, IL-1β, IL-2, IL-6, IL-8, IL-12p70, TNF-α) compared to HCWs LTB− (p < [0.0001–0.01]). Secretion of anti-inflammatory/Th2 cytokines IL-5 and IL-10 was also higher in HCWs LTB+ than HCWs LTB− . In conclusion, LTBI individuals controlling the M . tuberculosis infection have an enhanced TB specific Th1-cytokines/proinflammatory response combined with selected Th2 type/anti-inflammatory cytokines induction.