Isolation and Characterization of a cDNA Clone for the Complete Protein Coding Region of the δ Subunit of the Mouse Acetylcholine Receptor

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 81; no. 24; pp. 7970 - 7974
• Main Authors: LaPolla, Robert J., Mayne, Katharine Mixter, Davidson, Norman
• Format: Journal Article
• Language: English
• Published: United States National Academy of Sciences of the United States of America 01.12.1984; National Acad Sciences
• Subjects: Amino Acid Sequence; Amino acids; Animals; Base Sequence; Cell Line; Cloning, Molecular; Complementary DNA; DNA; DNA - isolation & purification; DNA Restriction Enzymes; Gels; Genes; Macromolecular Substances; Messenger RNA; Mice; Mice, Inbred C3H; Nucleic acids; Nucleotide sequences; Nucleotides; Open reading frames; Receptors, Cholinergic - genetics; RNA; RNA, Messenger - genetics; Species Specificity; Torpedo
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.81.24.7970

A mouse cDNA clone has been isolated that contains the complete coding region of a protein highly homologous to the δ subunit of the Torpedo acetylcholine receptor (AcChoR). The cDNA library was constructed in the vector λ gt10 from membrane-associated poly(A)+RNA from BC3H-1 mouse cells. Surprisingly, the δ clone was selected by hybridization with cDNA encoding the γ subunit of the Torpedo AcChoR. The nucleotide sequence of the mouse cDNA clone contains an open reading frame of 520 amino acids. This amino acid sequence exhibits 59% and 50% sequence homology to the Torpedo AcChoR δ and γ subunits, respectively. However, the mouse nucleotide sequence has several stretches of high homology with the Torpedo γ subunit cDNA, but not with δ . The mouse protein has the same general structural features as do the Torpedo subunits. It is encoded by a 3.3-kilobase mRNA. There is probably only one, but at most two, chromosomal genes coding for this or closely related sequences.