Isolation and Characterization of a cDNA Clone for the Complete Protein Coding Region of the δ Subunit of the Mouse Acetylcholine Receptor
A mouse cDNA clone has been isolated that contains the complete coding region of a protein highly homologous to the δ subunit of the Torpedo acetylcholine receptor (AcChoR). The cDNA library was constructed in the vector λ gt10 from membrane-associated poly(A)+RNA from BC3H-1 mouse cells. Surprising...
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Published in | Proceedings of the National Academy of Sciences - PNAS Vol. 81; no. 24; pp. 7970 - 7974 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
United States
National Academy of Sciences of the United States of America
01.12.1984
National Acad Sciences |
Subjects | |
Online Access | Get full text |
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Summary: | A mouse cDNA clone has been isolated that contains the complete coding region of a protein highly homologous to the δ subunit of the Torpedo acetylcholine receptor (AcChoR). The cDNA library was constructed in the vector λ gt10 from membrane-associated poly(A)+RNA from BC3H-1 mouse cells. Surprisingly, the δ clone was selected by hybridization with cDNA encoding the γ subunit of the Torpedo AcChoR. The nucleotide sequence of the mouse cDNA clone contains an open reading frame of 520 amino acids. This amino acid sequence exhibits 59% and 50% sequence homology to the Torpedo AcChoR δ and γ subunits, respectively. However, the mouse nucleotide sequence has several stretches of high homology with the Torpedo γ subunit cDNA, but not with δ . The mouse protein has the same general structural features as do the Torpedo subunits. It is encoded by a 3.3-kilobase mRNA. There is probably only one, but at most two, chromosomal genes coding for this or closely related sequences. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0027-8424 1091-6490 |
DOI: | 10.1073/pnas.81.24.7970 |