Research Note: A novel recombinant subgroup E isolate of the avian leukosis virus with a subgroup B-like Gp85 region in China

• Published in: Poultry science Vol. 100; no. 7; p. 101137
• Main Authors: Li, Jing, Liu, Lanlan, Niu, Xinxin, Li, Jiukuan, Kang, Zhonghui, Han, Chunyan, Gao, Yulong, Qi, Xiaole, Wang, Yongqiang, Wang, Xiaomei, Zeng, Xiangwei
• Format: Journal Article
• Language: English
• Published: Elsevier Inc 01.07.2021; Elsevier
• Subjects: ALV-B; ALV-E; Avian leukosis virus; China; farms; genes; gp85; IMMUNOLOGY, HEALTH AND DISEASE; mutational analysis; phylogeny; point mutation; poultry science; reorganization; viruses; China; ALV-E; reorganization; gp85; ALV-B
• ISSN: 0032-5791; 1525-3171; 1525-3171
• DOI: 10.1016/j.psj.2021.101137

The avian leukosis virus (ALV) strain DL00766 was isolated from a farm in China. The phylogenetic analysis showed that env had the highest homology with the E subgroup reference strain, ranging from 94.5% to 94.9%, whereas gp85 had the highest homology with the B and E subgroups, which were 89.0% to 91.3% and 91.3% to 91.8%. In addition, point mutation analysis of gp85 showed that a 400 bp long fragment in gp85 of DL00766 had the highest homology with subgroup B, ranging from 90.1% to 97.5%, and only 82.7% to 83.1% with E subgroup. These results indicate, DL00766 may be an AVL subgroup E isolate with a subgroup B-like gp85 region. This is also the first finding that the E subgroup is used as a recombinant subject, and the subgroup B provides a recombinant virus of an exogenous gene.