Aloe djiboutiensis : Antioxidant Activity, Molecular Networking-Based Approach and In Vivo Toxicity of This Endemic Species in Djibouti

• Published in: Molecules (Basel, Switzerland) Vol. 26; no. 10; p. 3046
• Main Authors: Elmi, Abdirahman, Mohamed Abdoul-Latif, Fatouma, Spina, Rosella, Dupire, François, Philippot, Stéphanie, Marie-France, Champy, Jacobs, Hugues, Laurain-Mattar, Dominique
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 20.05.2021; MDPI
• Subjects: Abnormalities; Aloe - chemistry; Aloe djiboutiensis; Analytical chemistry; Animals; Anthraquinones; antioxidant; Antioxidants; Antioxidants - pharmacology; Biocompatibility; Body Temperature - drug effects; Calcium; Chemical Sciences; Chromatography, Liquid - methods; Djibouti; Dry weight; Endemic species; Female; Flavonoids; Flavonols; Gallic acid; Isomers; Latex; Liquid chromatography; Male; Mass spectrometry; Mass spectroscopy; Metabolites; Mice; molecular networking; Natural products; Networking; NMR; Nuclear magnetic resonance; Organic chemistry; Phytochemicals; Plant Extracts - administration & dosage; Plant Extracts - pharmacology; Polyphenols; Quercetin; Scavenging; Scientific imaging; Social organization; Sulfonic acid; Tandem Mass Spectrometry - methods; Toxicity; Websites; Djibouti; United States > US; polyphenols; antioxidant; molecular networking; Aloe djiboutiensis; serum enzymes; histopathology; mass spectrometry; anthraquinones; cytotoxicity
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules26103046

For the first time, the study of the antioxidant activity, the characterization of the phytoconstituants, and the evaluation of in vitro and in vivo toxicity of leave and latex are performed. The antioxidant activity of both latex (ADL) and the methanolic extract of leaves (ADM) is determined using 1-diphenyl-2-picrylhydrazyl (DPPH), 2,2'-azino-bis 3-ethylbenzothiazoline-6-sulphonic acid (ABTS) scavenging radical methods and ferric reducing/antioxidant power (FRAP) assay. The phytochemical study of latex is done using Liquid Chromatography-Mass Spectrometry (LC-MS/MS) and a molecular networking-based approach. The evaluation of in vivo toxicity is performed on mice by oral gavage with a suspension of ADL. Our results show that weak antioxidant activity of ADL and ADM in opposition to their high polyphenol, 83.01 mg and 46.4 mg expressed in gallic acid equivalent (GAE)/g of dry weight (DW), respectively, and flavonoid contents 13.12 mg and 4.25 mg expressed in quercetin equivalent (QE)/g dry weight (DW), respectively. Using the Global Natural Products Social Molecular Networking (GNPS) website, nine (9) anthraquinones derivatives, ten (10) chromones derivatives, two (2) flavonols/ chromones isomers are annotated in the molecular network. The treated mice do not display abnormalities in their general physical appearance and biochemistry parameters, compared to the controls. Only glucose and calcium levels are slightly higher in male treated mice compared to the vehicles.