A comparison of methods for calculating DNA double-strand break induction frequency in mammalian cells by pulsed-field gel electrophoresis

Pulsed-field electrophoresis (PFGE) has become one of the most widely used methods for the evaluation of radiation-induced DNA double-strand breaks (dsb). In most studies a simple quantification of DNA migration from the well in the gel has been used as the correlate with dsb formation. Here we have...

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Bibliographic Details
Published inInternational journal of radiation biology Vol. 65; no. 6; p. 641
Main Authors Ruiz de Almodóvar, J M, Steel, G G, Whitaker, S J, McMillan, T J
Format Journal Article
LanguageEnglish
Published England 1994
Subjects
Cobalt Radioisotopes
DNA Damage
DNA, Neoplasm - radiation effects
Electrophoresis, Gel, Pulsed-Field
Humans
Hypoxia
In Vitro Techniques
Iodine Radioisotopes
Tumor Cells, Cultured
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Summary:Pulsed-field electrophoresis (PFGE) has become one of the most widely used methods for the evaluation of radiation-induced DNA double-strand breaks (dsb). In most studies a simple quantification of DNA migration from the well in the gel has been used as the correlate with dsb formation. Here we have compared such a method, as calibrated with 125I-labelled UdR, with two methods which involved the analysis of the distribution of sizes of DNA fragments migrating in the gel. We conclude that the three methods produce similar absolute values for dsb induction frequency. It is not clear which is the single method of choice but the comparison of the analyses increases the information which can be derived from PFGE experiments.
ISSN:0955-3002
DOI:10.1080/09553009414550751