Human Cytomegalovirus-Encoded miR-US25-1 Aggravates the Oxidised Low Density Lipoprotein-Induced Apoptosis of Endothelial Cells

• Published in: BioMed research international Vol. 2014; no. 2014; pp. 1 - 13
• Main Authors: Fan, Jianmin, Zhang, Wen, Liu, Qiming
• Format: Journal Article
• Language: English
• Published: Cairo, Egypt Hindawi Puplishing Corporation 01.01.2014; Hindawi Publishing Corporation; John Wiley & Sons, Inc
• Subjects: 5' Untranslated Regions; Apoptosis; Apoptosis - genetics; Atherosclerosis; Atherosclerosis - genetics; Atherosclerosis - pathology; Atherosclerosis - virology; Causes of; Cell cycle; Cytomegalovirus; Cytomegalovirus - genetics; Cytomegalovirus - pathogenicity; Cytomegalovirus Infections - genetics; Cytomegalovirus Infections - pathology; Cytomegalovirus Infections - virology; Cytomegaloviruses; Development and progression; Endothelial Cells - metabolism; Endothelial Cells - pathology; Endothelial Cells - virology; Endothelium; Gene expression; Genetic aspects; Health aspects; Human cytomegalovirus; Humans; Infections; Lipoproteins, LDL - metabolism; Low density lipoproteins; MicroRNA; MicroRNAs - genetics; Polyclonal antibodies; Proteins; Smooth muscle; Traditional Chinese medicine
• ISSN: 2314-6133; 2314-6141; 2314-6141
• DOI: 10.1155/2014/531979

Human cytomegalovirus (HCMV) infection is linked to the development and severity of the cardiovascular disease atherosclerosis; however, there is little known about the promotion of atherosclerosis. miR-US25-1 is one of HCMV-encoded miRNAs and targets cellular genes that are essential for virus growth to control the life cycle of the virus and host cells. The prominent regulation on cell cycle genes of the miR-US25-1 attracts us to explore its role in the atherosclerosis promotion. It was indicated that miR-US25-1 level was upregulated in subjects or in endothelial cells with HCMV infection; and the miR-US25-1 downregulated the expression of BRCC 3 by targeting the 5′ UTR of BRCC 3. And a miR-US25-1 mimics transfection could reduce the EAhy926 cell viability but did not induce apoptosis in EAhy926 cells. And what is more, miR-US25-1 mimicis transfection deteriorated the ox-LDL-induced apoptosis and aggravated the upregulation of apoptosis-associated molecules by oxidised low density lipoprotein (ox-LDL) in EAhy926 cells. And we have also confirmed the deregulation of BRCC 3 expression by miR-US25-1 by targeting the 5′ UTR of it. Given the vital role of BRCC 3 in DNA damage repairing, we speculated that the targeting inhibition of BRCC 3 by miR-US25-1 may contribute to the aggravation of ox-LDL-promoted apoptosis of endothelial EAhy926 cells.