Porcine Pituitary Dynorphin: Complete Amino Acid Sequence of the Biologically Active Heptadecapeptide

The full primary structure of the very potent opioid peptide dynorphin, from porcine pituitary, has been determined. It is (H)Tyr-Gly-Gly-Phe-Leu-Arg-Arg-Ile-Arg-Pro-Lys-Leu-Lys-Trp-Asp-Asn-Gln(OH). The synthetic peptide with this sequence behaves identically to natural dynorphin in a number of ways...

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Published inProceedings of the National Academy of Sciences - PNAS Vol. 78; no. 11; pp. 7219 - 7223
Main Authors Goldstein, Avram, Fischli, Walter, Lowney, Louise I., Hunkapiller, Michael, Hood, Leroy
Format Journal Article
LanguageEnglish
Published United States National Academy of Sciences of the United States of America 01.11.1981
National Acad Sciences
Subjects
Amino Acid Sequence
Amino acids
Animals
Bioassay
Carbonates
Dynorphins
Endorphins - isolation & purification
Opioid peptides
Peptide Fragments - analysis
Pituitary Gland - analysis
Precipitates
Quaternary ammonium compounds
Radioimmunoassay
Resins
Sequence analysis
Swine
Trypsin
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Summary:The full primary structure of the very potent opioid peptide dynorphin, from porcine pituitary, has been determined. It is (H)Tyr-Gly-Gly-Phe-Leu-Arg-Arg-Ile-Arg-Pro-Lys-Leu-Lys-Trp-Asp-Asn-Gln(OH). The synthetic peptide with this sequence behaves identically to natural dynorphin in a number of ways, and it has the same potency in the guinea pig ileum myenteric plexus--longitudinal muscle bioassay. The potency is accounted for by the first 13 residues.
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ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.78.11.7219