Over-expression of programmed death-ligand 1 and programmed death-1 on antigen-presenting cells as a predictor of organ dysfunction and mortality during early sepsis: a prospective cohort study

• Published in: World journal of emergency medicine Vol. 14; no. 3; pp. 179 - 185
• Main Authors: Li, Jia-Bao, Xie, Miao-Rong, Duan, Mei-Li, Yu, Ya-Nan, Hang, Chen-Chen, Tang, Zi-Ren, Li, Chun-Sheng
• Format: Journal Article
• Language: English
• Published: China World Journal of Emergency Medicine 01.01.2023; Emergency and Critical Care Center Beijing Friendship Hospital,Capital Medical University,Beijing 100050,China%Emergency and Critical Care Center Beijing Friendship Hospital,Capital Medical University,Beijing 100050,China%Department of Emergency Medicine,Beijing Chaoyang Hospital,Capital Medical University,Beijing 100020,China; Department of Critical Care Medicine,Beijing Friendship Hospital,Capital Medical University,Beijing 100050,China; Second Affiliated Hospital of Zhejiang University School of Medicine
• Subjects: B cells; China; Cytokines; Dendritic cells; Ethylenediaminetetraacetic acid; Health aspects; Immunity; Mortality; Original; China; Inflammation; Programmed death-ligand 1; Antigen-presenting cells; Programmed death-1
• ISSN: 1920-8642
• DOI: 10.5847/wjem.j.1920-8642.2023.041

This study aimed to explore the changes of programmed death-ligand 1 (PD-L1) and programmed death-1 (PD-1) expression on antigen-presenting cells (APCs) and evaluate their association with organ failure and mortality during early sepsis. In total, 40 healthy controls and 198 patients with sepsis were included in this study. Peripheral blood was collected within the first 24 h after the diagnosis of sepsis. The expression of PD-L1 and PD-1 was determined on APCs, such as B cells, monocytes, and dendritic cells (DCs), by flow cytometry. Cytokines in plasma, such as interferon-γ (IFN-γ), tumor necrosis factor-α (TNF-α), interleukin-4 (IL-4), IL-6, IL-10, and IL-17A were determined by Luminex assay. PD-1 expression decreased significantly on B cells, monocytes, myeloid DCs (mDCs), and plasmacytoid DCs (pDCs) as the severity of sepsis increased. PD-1 expression was also markedly decreased in non-survivors compared with survivors. In contrast, PD-L1 expression was markedly higher on mDCs, pDCs, and monocytes in patients with sepsis than in healthy controls and in non-survivors than in survivors. The PD-L1 expression on APCs (monocytes and DCs) was weakly related to organ dysfunction and inflammation. The area under the receiver operating characteristic curve (AUC) of the PD-1 percentage of monocytes (monocyte PD-1%)+APACHE II model (0.823) and monocyte PD-1%+SOFA model (0.816) had higher prognostic value than other parameters alone. Monocyte PD-1% was an independent risk factor for 28-day mortality. The severity of sepsis was correlated with PD-L1 or PD-1 over-expression on APCs. PD-L1 in monocytes and DCs was weakly correlated with inflammation and organ dysfunction during early sepsis. The combination of SOFA or APACHE II scores with monocyte PD-1% could improve the prediction ability for mortality.