Transcription factors AS1 and AS2 interact with LHP1 to repress KNOX genes in Arabidopsis

• Published in: Journal of integrative plant biology Vol. 58; no. 12; pp. 959 - 970
• Main Authors: Li, Zhongfei, Li, Bin, Liu, Jian, Guo, Zhihao, Liu, Yuhao, Li, Yan, Shen, Wen-Hui, Huang, Ying, Huang, Hai, Zhang, Yijing, Dong, Aiwu
• Format: Journal Article
• Language: English
• Published: China (Republic : 1949- ) Blackwell Publishing Ltd 01.12.2016; State Key Laboratory of Genetic Engineering, International Associated Laboratory of CNRS-Fudan-HUNAU on Plant Epigenome Research, Col aborative Innovation Center for Genetics and Development, Institute of Plant Biology, School of Life Sciences, Fudan University, Shanghai 200433, China%National Laboratory of Plant Molecular Genetics, Shanghai Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China%National Center for Protein Science Shanghai, State Key Laboratory of Molecular Biology, Shanghai State Key Laboratory of Molecular Andrology, Institute of Biochemistry and Cel Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 201203, China; Wiley
• Subjects: Arabidopsis; Arabidopsis - genetics; Arabidopsis - metabolism; Arabidopsis Proteins - genetics; Arabidopsis Proteins - metabolism; Arabidopsis thaliana; ASYMMETRIC LEAVES 1; ASYMMETRIC LEAVES 2; Biocatalysis; Biochemistry, Molecular Biology; Cellular Biology; Class-I KNOX genes; Epistasis, Genetic; eukaryotic cells; flowers; Gene Expression Regulation, Plant; genes; Genes, Plant; Genetic Loci; H3K27me3 establishment; heterochromatin; histones; Histones - metabolism; Homeodomain Proteins - genetics; Homeodomain Proteins - metabolism; leaf development; leaves; Life Sciences; LIKE HETEROCHROMATIN PROTEIN 1; lysine; Lysine - metabolism; Methylation; Molecular biology; Phenotype; Plant Leaves - genetics; Protein Binding; Repressor Proteins - metabolism; Reproducibility of Results; RNA, Messenger - genetics; RNA, Messenger - metabolism; transcription (genetics); transcription factors; Transcription Factors - metabolism; transcriptomics; Vegetal Biology; Arabidopsis thaliana; LIKE HETEROCHROMATIN PROTEIN 1; ASYMMETRIC LEAVES 1; ASYMMETRIC LEAVES 2; Class-I KNOX genes; H3K27me3 establishment
• ISSN: 1672-9072; 1744-7909
• DOI: 10.1111/jipb.12485

Polycomb group proteins are important repressors of numerous genes in higher eukaryotes. However, the mechanism by which Polycomb group proteins are recruited to specific genes is poorly understood. In Arabidopsis, LIKE HETEROCHROMATIN PROTEIN 1 （LHP1）, also known as TERMINAL FLOWER 2, was originally proposed as a subunit of polycomb repressive complex 1 （PRC1） that could bind the tri-methylated lysine 27 of histone H3 （H3K27me3） established by the PRC2. In this work, we show that LHP1 mainly functions with PRC2 to establish H3K27me3, but not with PRC1 to catalyze monoubiquitination at lysine 119 of histone H2A. Our results show that complexes of the transcription factors ASYMMETRIC LEAVES 1 （AS1） and AS2 could help to establish the H3K27me3 modification at the chromatin regions of Class-I KNOTTED＇t-like homeobox （KNOX） genes BREVIPEDICELLU5 and KNAT2 via direct interactions with LHP1. Additionally, our transcriptome analysis indicated that there are probably more common target genes of AS1 and LHP1 besides Class-I KNOX genes during leaf development in Arabidopsis.