Chromosomal reallocation of the chicken c-myb locus and organization of 3′-proximal coding exons

• Published in: FEBS letters Vol. 263; no. 2; pp. 254 - 260
• Main Authors: Soret, Johann, Vellard, Michel, Viegas-Pequignot, Evani, Apiou, Françoise, Dutrillaux, Bernard, Perbal, Bernard
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 24.04.1990; Elsevier
• Subjects: Animals; Avian genome; Base Sequence; Biological and medical sciences; Blotting, Southern; c-myb proto-oncogene; Chickens - genetics; Chromosome Mapping; Coding exon; DNA - genetics; DNA Probes; Exons; Fundamental and applied biological sciences. Psychology; Genes. Genome; Lymphoma; Molecular and cellular biology; Molecular genetics; Molecular Sequence Data; Nucleic Acid Hybridization; Proto-Oncogene Proteins - genetics; Proto-Oncogene Proteins c-myb; Splicing; c-myb proto-oncogene; Chromosome mapping; Coding exon; Splicing; Lymphoma; Avian genome; Molecular hybridization; Vertebrata; Nucleotide sequence; C-Onc gene; In situ; Chromosome; Gene organization; Chicken; Molecular probe; Aves; Localization
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/0014-5793(90)81387-4

In the course of our studies concerning the tissue-specific expression of the c-myb proto-oncogene, we have established the nucleotide sequence of the chicken c-myb 3′-proximal coding exons. In situ hybridization performed with different genomic DNA probes corresponding to nearly all the c-myb gene allowed us to localize the corresponding locus on the large acrocentric chromosome 3 in chicken. Our sequencing data also indicate that the 3′-proximal noncoding sequences represented in c-myb mRNA species are derived from non-contiguous exons.