Comparative Molecular Analysis of Haemophilus influenzae Isolates from Young Children with Acute Lower Respiratory Tract Infections and Meningitis in Hanoi, Vietnam

• Published in: Journal of Clinical Microbiology Vol. 43; no. 5; pp. 2474 - 2476
• Main Authors: Watanabe, Hiroshi, Kaji, Chiharu, Anh, Dang Duc, Huong, Phan Le Thanh, Anh, Nguyen Thi Hien, Huong, Vu Thi, Phuong, Hoang Vu Mai, Thi, Ngo Thi, Suu, Pham Thi, Nguyet, Nguyen Thi Thu, Rusizoka, Olivia Sebastian, Watanabe, Kiwao, Nagatake, Tsuyoshi, Oishi, Kazunori
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for Microbiology 01.05.2005
• Subjects: Antibodies, Bacterial - blood; Bacteriology; Biological and medical sciences; Child; Child, Preschool; DNA, Bacterial - genetics; DNA, Bacterial - isolation & purification; Electrophoresis, Gel, Pulsed-Field - methods; Epidemiology; Fundamental and applied biological sciences. Psychology; Haemophilus Infections - diagnosis; Haemophilus influenzae; Haemophilus influenzae - genetics; Haemophilus influenzae - isolation & purification; Humans; Infant; Infectious diseases; Medical sciences; Meningitis, Bacterial - diagnosis; Microbiology; Miscellaneous; Respiratory Tract Infections - diagnosis; Restriction Mapping; Asia; Vietnam; Human; Lung disease; Nervous system diseases; Pneumonia; Microbiology; Respiratory disease; Acute; Haemophilus influenzae; Pasteurellaceae; Central nervous system disease; Bacteria; Isolate; Meningitis; Child
• ISSN: 0095-1137; 1098-660X
• DOI: 10.1128/JCM.43.5.2474-2476.2005

Thirty-seven Haemophilus influenzae strains from nasopharyngeal swabs (NP) and 44 H. influenzae strains from cerebrospinal fluid (CSF) were investigated. Of the 37 H. influenzae isolates from NP, the serotypes of 30 isolates were nontypeable, 4 were type b, 2 were type c, and 1 was type a, whereas all of the 44 isolates from CSF were type b. The MICs of 16 antibiotics for the H. influenzae isolates from NP and CSF were similar, and no {szligbeta}-lactamase-negative ampicillin-resistant strain was found. Molecular typing by pulsed-field gel electrophoresis (PFGE) showed that the 37 H. influenzae strains from NP had 22 PFGE patterns, with none predominating, and the 44 H. influenzae strains from CSF had 9 PFGE patterns, with patterns [alpha] (22 isolates) and {szligbeta} (12 isolates) predominating. Our results indicate that two predominant types of H. influenzae type b strains have the potential to spread among children with meningitis in Hanoi, Vietnam.