Increased apelin receptor gene expression in the subfornical organ of spontaneously hypertensive rats

• Published in: PloS one Vol. 15; no. 4; p. e0231844
• Main Authors: Griffiths, Philip R, Lolait, Stephen J, Bijabhai, Aarifah, O'Carroll-Lolait, Aoife, Paton, Julian F R, O'Carroll, Anne-Marie
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 21.04.2020; Public Library of Science (PLoS)
• Subjects: Animals; Apelin - metabolism; Apelin Receptors - metabolism; Area postrema; Autonomic nervous system; Biology and Life Sciences; Blood pressure; Blood-brain barrier; Brain; Experiments; Gene expression; Health sciences; Histochemistry; Histology; Homeostasis; Hybridization; Hypertension; Hypertension - metabolism; Hypertension - pathology; In Situ Hybridization, Fluorescence; Level (quantity); Male; Medical schools; Medicine and Health Sciences; Microinjection; Neuronal-glial interactions; Neurons - metabolism; Neurons - pathology; Organs; Phenotypes; Physiology; Rats; Rats, Inbred SHR; Rats, Inbred WKY; Receptors; Research and Analysis Methods; Rodents; Sense organs; Signal transduction; Subfornical organ; Subfornical Organ - metabolism; Subfornical Organ - pathology; Up-Regulation; United Kingdom > UK
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0231844

The vascular organ of the lamina terminalis, subfornical organ (SFO), and area postrema comprise the sensory circumventricular organs (CVO) which are central structures that lie outside the blood brain barrier and are thought to provide an interface between peripherally circulating signals and the brain through their projections to central autonomic structures. The SFO expresses mRNA for the G protein-coupled apelin receptor (APJ, gene name aplnr) and exogenous microinjection of the neuropeptide apelin (apln) to the SFO elicits a depressor effect. Here we investigated the expression and cellular distribution of aplnr, apln and the recently described ligand apela (apela) in the CVOs and investigated whether differences in the levels of expression of apelinergic gene transcripts in these regions might underlie the chronic elevated blood pressure seen in hypertension. We carried out multiplex in situ hybridization histochemistry on CVO tissue sections from spontaneously hypertensive rats (SHR) and normotensive Wistar Kyoto (WKY) controls. Confocal immunofluorescent images indicated strong aplnr expression, with lower levels of apln and modest apela expression, in the CVOs of both WKY rats and SHRs, in both neurons and glia. The expression level of aplnr transcripts was increased in the SFO of SHRs compared to WKY rats. Our data may highlight a potential dysfunction in the communication between CVOs and downstream signalling pathways in SHRs, which may contribute to its different phenotype/s.