Chlorogenic acid inhibits the replication and viability of enterovirus 71 in vitro

• Published in: PloS one Vol. 8; no. 9; p. e76007
• Main Authors: Li, Xiang, Liu, Yuanyuan, Hou, Xueling, Peng, Hongjun, Zhang, Li, Jiang, Qingbo, Shi, Mei, Ji, Yun, Wang, Yuyue, Shi, Weifeng
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 30.09.2013; Public Library of Science (PLoS)
• Subjects: Acids; Antiviral activity; Apoptosis; Chemokine CCL2 - metabolism; Chlorogenic acid; Chlorogenic Acid - chemistry; Chlorogenic Acid - pharmacology; Cytokines; Cytotoxicity; Electrophoresis, Polyacrylamide Gel; Enterovirus A, Human - drug effects; Enterovirus Infections - drug therapy; Enteroviruses; Etiology; Gene Expression Regulation, Viral - drug effects; Herbal medicine; Humans; Infections; Inhibitory Concentration 50; Interferon; Interferon-gamma - metabolism; Interleukin 6; Interleukin-6 - metabolism; Laboratories; Microbial Viability - drug effects; Molecular Structure; Monocyte chemoattractant protein 1; Replication; Secretions; Time Factors; Transcription; Tumor Necrosis Factor-alpha - metabolism; Tumor necrosis factor-α; Viability; Viral infections; Virus Replication - drug effects; Viruses; γ-Interferon; China
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0076007

Enterovirus 71 (EV71) is an etiology for a number of diseases in humans. Traditional Chinese herbs have been reported to be effective for treating EV71 infection. However, there is no report about the antiviral effects of CHA against EV71. In this study, plaque reduction assay demonstrated that the inhibitory concentration 50% (IC50) of CHA on EV71 replication is 6.3 µg/ml. When both CHA (20 µg/ml) and EV71 were added, or added post-infection at different time points, CHA was able to effectively inhibit EV71 replication between 0 and 10 h. In addition, CHA inhibited EV71 2A transcription and translation in EV71-infected RD cells, but did not affect VP1, 3C, and 3D expression. Furthermore, CHA inhibited secretions of IL-6, TNF-α, IFN-γ and MCP-1 in EV71-infected RD cells. Altogether, these results revealed that CHA may have antiviral properties for treating EV71 infection.