Regulated expression of PTPRJ/CD148 and an antisense long noncoding RNA in macrophages by proinflammatory stimuli

• Published in: PloS one Vol. 8; no. 6; p. e68306
• Main Authors: Dave, Richa K, Dinger, Marcel E, Andrew, Megan, Askarian-Amiri, Marjan, Hume, David A, Kellie, Stuart
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 28.06.2013; Public Library of Science (PLoS)
• Subjects: Animals; Antisense RNA; Base Sequence; Binding sites; Biology; Cancer; Cell Line; Cytokines; Dendritic cells; Ethics; Gene expression; Gene loci; Growth factors; HEK293 Cells; Humans; Immune system; Immunohistochemistry; Immunology; Infectious diseases; Inflammation; Inflammation - genetics; Inflammation - metabolism; Inflammatory diseases; Interferon; Kinases; Ligands; Lipopolysaccharides; Lymphoid tissue; Macrophage colony-stimulating factor; Macrophage Colony-Stimulating Factor - genetics; Macrophage Colony-Stimulating Factor - metabolism; Macrophages; Macrophages - metabolism; Male; Mice; Mice, Inbred C57BL; Molecular Sequence Data; NIH 3T3 Cells; Phagocytes - metabolism; Phosphatase; Phosphorylation; Protein-tyrosine-phosphatase; Proteins; Receptor-Like Protein Tyrosine Phosphatases, Class 3 - genetics; Receptor-Like Protein Tyrosine Phosphatases, Class 3 - metabolism; Ribonucleic acid; RNA; RNA, Messenger - genetics; RNA, Messenger - metabolism; RNA, Untranslated - genetics; RNA, Untranslated - metabolism; Tissues; Toll-like receptors; Toll-Like Receptors - genetics; Toll-Like Receptors - metabolism; Tumors; Tyrosine; Australia
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0068306

PTPRJ/CD148 is a tyrosine phosphatase that has tumour suppressor-like activity. Quantitative PCR of various cells and tissues revealed that it is preferentially expressed in macrophage-enriched tissues. Within lymphoid tissues immunohistochemistry revealed that PTPRJ/CD148 co-localised with F4/80, indicating that macrophages most strongly express the protein. Macrophages express the highest basal level of ptprj, and this is elevated further by treatment with LPS and other Toll-like receptor ligands. In contrast, CSF-1 treatment reduced basal and stimulated Ptprj expression in human and mouse cells, and interferon also repressed Ptprj expression. We identified a 1006 nucleotide long noncoding RNA species, Ptprj-as1 that is transcribed antisense to Ptprj. Ptprj-as1 was highly expressed in macrophage-enriched tissue and was transiently induced by Toll-like receptor ligands with a similar time course to Ptprj. Finally, putative transcription factor binding sites in the promoter region of Ptprj were identified.