Myo-inositol hexakisphosphate degradation by Bifidobacterium infantis ATCC 15697

• Published in: International journal of food microbiology Vol. 117; no. 1; pp. 76 - 84
• Main Authors: Haros, Monica, Bielecka, Maria, Honke, Joanna, Sanz, Yolanda
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 10.06.2007; Elsevier
• Subjects: 6-Phytase - metabolism; antinutritional factors; Bifidobacterium - enzymology; Bifidobacterium - growth & development; Bifidobacterium - metabolism; Bifidobacterium animalis; Bifidobacterium infantis; Bifidobacterium longum; Bifidobacterium pseudolongum; biodegradation; Biological and medical sciences; culture media; Food industries; Food Microbiology; food processing; Fundamental and applied biological sciences. Psychology; gastrointestinal transit; Hydrogen-Ion Concentration; Inositol Phosphates - metabolism; Inositol-phosphates; Kinetics; myo-inositol; phosphates; Phytate-degrading enzyme; Phytic acid; Phytic Acid - metabolism; Probiotics; Temperature; Inositol-phosphates; Phytate-degrading enzyme; Phytic acid; Bifidobacterium infantis; Phosphates; Bifidobacterium; Actinomycetaceae; Enzyme; Actinomycetales; Degradation; Antinutrient factor; Bacteria; Actinomycetes
• ISSN: 0168-1605; 1879-3460
• DOI: 10.1016/j.ijfoodmicro.2007.02.021

The ability of five strains of different bifidobacterial species ( Bifidobacterium animalis, B. bifidum, B. infantis, B. longum, B. pseudolongum) isolated from human and animal gut to degrade myo-inositol hexaphosphate or phytic acid (InsP 6) has been evaluated. The disappearance of phytate and the generation of lower myo-inositol phosphates were determined in a complex medium in which phytic acid was the only source of phosphorus. Bifidobacterium infantis ATCC 15697 showed the highest level of phytate-degrading activity. This strain displayed optimal activity at slight acid pH (6.0–6.5) and 50 °C, but also retained high activity levels at neutral pH and 37 °C. B. infantis ATCC 15697 produced maximum activity during the stationary phase of growth and when 1% lactose was used as carbon source. In contrast, the presence of inorganic phosphate in the growth medium inhibited phytase activity. The ability of B. infantis ATCC 15697 to generate lower myo-inositol phosphates and, particularly, accumulate myo-inositol tri-phosphates (InsP 3) from partial hydrolysis of InsP 6 could contribute to the reduction of the anti-nutritional properties of InsP 6 and the generation of intermediate compounds with beneficial properties during food processing and gastrointestinal transit.