Modification of a Mammalian Cell Protein in the Presence of [³²P-Adenylate]NAD: Evidence for ADP Ribosylation Activity Associated with Helicobacter pylori

Culture filtrates from Helicobacter pylori promote the transfer of the radiolabel from [³²P-adenylate]NAD to one or more heat-labile factors within extracts prepared from several mammalian cell lines, with the predominate radiolabeled species exhibiting an apparent molecular mass of greater than 130...

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Published inInfection and Immunity Vol. 74; no. 5; pp. 3071 - 3076
Main Authors Nossa, Carlos W, Blanke, Steven R
Format Journal Article
LanguageEnglish
Published Washington, DC American Society for Microbiology 01.05.2006
Subjects
ADP Ribose Transferases - metabolism
Bacteriology
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
HeLa Cells
Helicobacter pylori
Helicobacter pylori - enzymology
Humans
Microbiology
Miscellaneous
Molecular Pathogenesis
NAD - metabolism
Phosphorus Radioisotopes
Proteins - metabolism
Vertebrata
ADP ribosylation
Mammalia
Spirillales
Helicobacter pylori
Microbiology
Spirillaceae
Bacteria
Immunity
Protein
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Summary:Culture filtrates from Helicobacter pylori promote the transfer of the radiolabel from [³²P-adenylate]NAD to one or more heat-labile factors within extracts prepared from several mammalian cell lines, with the predominate radiolabeled species exhibiting an apparent molecular mass of greater than 130 kDa. Our results suggest that several H. pylori strains release a factor that ADP-ribosylates a mammalian target protein.
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Corresponding author. Mailing address: Department of Microbiology, B103 CLSL, University of Illinois, 601 South Goodwin Ave., Urbana, IL 61801. Phone: (217) 244-2412. Fax: (713) 743-8351. E-mail: sblanke@life.uiuc.edu.
Editor: D. L. Burns
ISSN:1098-5522
0019-9567
1098-5522
DOI:10.1128/IAI.74.5.3071-3076.2006