Development and Structure of Internal Glands and External Glandular Trichomes in Pogostemon cablin

• Published in: PloS one Vol. 8; no. 10; p. e77862
• Main Authors: Guo, Jiansheng, Yuan, Yongming, Liu, Zhixue, Zhu, Jian
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 2013; Public Library of Science (PLoS)
• Subjects: Biology; Biosynthesis; Cell division; Cell walls; Comparative analysis; Endoplasmic reticulum; Flavones; Flavones - analysis; Flavonoids; Glands; Histochemistry; Histocytochemistry; Histology; Lamiaceae - chemistry; Lamiaceae - cytology; Lamiaceae - ultrastructure; Leaves; Lentibulariaceae; Life sciences; Lipids; Lipids - analysis; Membranes; Microscopy, Electron, Scanning; Morphology; Oils & fats; Physiology; Plant Leaves - chemistry; Plant Leaves - cytology; Plant Leaves - ultrastructure; Plant Oils - chemistry; Plastids; Pogostemon cablin; Secretion; Secretions; Stems; Terpenes; Terpenes - analysis; Trichomes; Trichomes - chemistry; Trichomes - cytology; Trichomes - ultrastructure; Vacuoles; China; Sudan
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0077862

Pogostemon cablin possesses two morphologically and ontogenetically different types of glandular trichomes, one type of bristle hair on the surfaces of leaves and stems and one type of internal gland inside the leaves and stems. The internal gland originates from elementary meristem and is associated with the biosynthesis of oils present inside the leaves and stems. However, there is little information on mechanism for the oil biosynthesis and secretion inside the leaves and stems. In this study, we identified three kinds of glandular trichome types and two kinds of internal gland in the Pogostemon cablin. The oil secretions from internal glands of stems and leaves contained lipids, flavones and terpenes. Our results indicated that endoplasmic reticulum and plastids and vacuoles are likely involved in the biosynthesis of oils in the internal glands and the synthesized oils are transported from endoplasmic reticulum to the cell wall via connecting endoplasmic reticulum membranes to the plasma membrane. And the comparative analysis of the development, distribution, histochemistry and ultrastructures of the internal and external glands in Pogostemon cablin leads us to propose that the internal gland may be a novel secretory structure which is different from external glands.