Leveling Response Factors in the Electrospray Ionization Process Using a Heated Capillary Interface

Several investigators have observed a discrepancy in electrospray response of complementary strands from denatured DNA, which has been attributed to the difference in hydrophobicity between the two strands; the more hydrophobic species tend to have higher ion abundances. The implementation of a heat...

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Bibliographic Details
Published inJournal of the American Society for Mass Spectrometry Vol. 16; no. 5; pp. 772 - 778
Main Authors Frahm, Jennifer L., Muddiman, David C., Burke, Michael J.
Format Journal Article
LanguageEnglish
Published New York, NY Elsevier Inc 01.05.2005
Elsevier Science
Springer Nature B.V
Subjects
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Capillary Action
Deoxyribonucleic acid
DNA
DNA - analysis
DNA - chemistry
Dna, deoxyribonucleoproteins
Electrospraying
Equipment Design
Equipment Failure Analysis
Fundamental and applied biological sciences. Psychology
Hot Temperature
Humans
Hydrophobicity
Ionization
Ions
Mass spectrometry
Nucleic acids
Purines
Specimen Handling - instrumentation
Specimen Handling - methods
Spectrometry, Mass, Electrospray Ionization - instrumentation
Spectrometry, Mass, Electrospray Ionization - methods
Strands
Polymerase chain reaction
Gene amplification
Analysis method
Instrumental response
Heating
Thermal denaturation
Single stranded DNA
Hydrophobicity
Mass spectrometry
Electrospray
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Summary:Several investigators have observed a discrepancy in electrospray response of complementary strands from denatured DNA, which has been attributed to the difference in hydrophobicity between the two strands; the more hydrophobic species tend to have higher ion abundances. The implementation of a heated electrospray source has allowed us to “level” the electrospray response for two equimolar complementary strands with different hydrophobicities. As the temperature was increased, the ratio of ion abundances of the less hydrophobic noncoding strand to the more hydrophobic coding strand approached unity. Furthermore, the heated electrospray source was used to denature amplicons containing 7-deaza purines, which can be used to facilitate sequencing by mass spectrometry.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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ISSN:1044-0305
1879-1123
DOI:10.1016/j.jasms.2005.02.002