Natural co-infection of torque teno virus and porcine circovirus 2 in the reproductive apparatus of swine

• Published in: Research in veterinary science Vol. 92; no. 3; pp. 519 - 523
• Main Authors: Ritterbusch, Giseli A., Sa Rocha, Camila A., Mores, Nelson, Simon, Neide L., Zanella, Eraldo L., Coldebella, Arlei, Ciacci-Zanella, Janice R.
• Format: Journal Article
• Language: English
• Published: England Elsevier India Pvt Ltd 01.06.2012; Elsevier Limited
• Subjects: Animals; Boar; Circoviridae; Circovirus - isolation & purification; Deoxyribonucleic acid; DNA; DNA Virus Infections - veterinary; DNA Virus Infections - virology; Failure; Farms; Female; Genital Diseases, Female - veterinary; Genital Diseases, Female - virology; Genital Diseases, Male - veterinary; Genital Diseases, Male - virology; Hogs; Male; Motility; Pathogenesis; PCV2; Polymerase Chain Reaction - methods; Polymerase Chain Reaction - veterinary; Porcine circovirus 2; Porcine torque teno virus; Reproductive; Semen; Semen - virology; Sow; Sperm; Studies; Swine; Swine Diseases - virology; Torque teno virus - isolation & purification; Veterinary medicine; Viral infections; Spain; Boar; Porcine torque teno virus; Reproductive; PCV2; Sow; Semen
• ISSN: 0034-5288; 1532-2661
• DOI: 10.1016/j.rvsc.2011.04.001

This work aimed to detect and study natural co-infection of Circoviridae torque teno virus (TTV) and porcine circovirus 2 (PCV2) in the swine reproductive apparatus. Semen and organs from 17 boars were tested by nested and real-time PCR. PCV2 was amplified from semen (47%), lymph nodes (84.6%) and testicles (35.3%). TTV2 was amplified from 16/17 testis and 13/13 lymph nodes. TTV1 DNA was detected in fewer testicle samples (2/17), which were also TTV2 positive. Analyzed ovaries, follicular fluid and uteri of 83 culled sows showed TTV2, TTV1 and PCV2 from 49.3%, 30.1% and 6.0% of the sows, respectively. Sperm analysis indicated insignificant differences between PCV2 and TTVs positive and negative boars. The most frequent pathologic lesion in sows was endometritis (28.9%), but this was unassociated with PCV2 or TTVs detection. These findings question the importance of PCV2 and TTV2 natural co-infection in the pathology of porcine reproductive failures.