Simultaneous determination of thiamin and riboflavin in the sea urchin, Paracentrotus lividus, by high-performance liquid chromatography

• Published in: International journal of food sciences and nutrition Vol. 55; no. 4; pp. 259 - 263
• Main Authors: Rodriguez-Bernaldo de Quiros, A, Lopez-Hernandez, J, Simal-Lozano, J
• Format: Journal Article
• Language: English
• Published: Basingstoke Informa UK Ltd 01.06.2004; Taylor & Francis; Nature Publishing; Taylor & Francis Ltd
• Subjects: Animals; Aquatic life; Biological and medical sciences; chemical composition; Chromatography; Chromatography, High Pressure Liquid - methods; Echinodermata; Echinoidea; Feeding. Feeding behavior; food analysis; Food science; Fundamental and applied biological sciences. Psychology; gonads; high performance liquid chromatography; Marine; Nutrition; Paracentrotus lividus; riboflavin; Riboflavin - analysis; Sea Urchins - chemistry; shellfish; thiamin; Thiamine - analysis; Vertebrates: anatomy and physiology, studies on body, several organs or systems; Vitamin B; vitamin content; Performance evaluation; Human; Liquid; Paracentrotus lividus; Physical performance; Echinodermata; Riboflavin; Echinoidea; B-Vitamins; Determination; Invertebrata; Thiamine
• ISSN: 0963-7486; 1465-3478
• DOI: 10.1080/09637480310001622323

Thiamin and riboflavin were determined in the sea urchin, Paracentrotus lividus, by reversed-phase liquid chromatography with fluorescence detection and a mobile phase consisting of 72% 0.005 M NH4OAc and 28% MeOH at a flow rate of 1.450 ml/min. The total analysis time never exceeded 20 min. After acid hydrolysis and enzymatic digestion, thiamin was oxidized to thiochrome and the oxidized fraction was purified and concentrated by solid-phase extraction. Limits of detection were 9 pg/ml and 80 pg/ml for thiamin and riboflavin, respectively. Good results were obtained with respect to repeatability (RSD%<2.4) and recovery (>91.3%).