QTL mapping based on different genetic systems for essential amino acid contents in cottonseeds in different environments

• Published in: PloS one Vol. 8; no. 3; p. e57531
• Main Authors: Liu, Haiying, Quampah, Alfred, Chen, Jinhong, Li, Jinrong, Huang, Zhuangrong, He, Qiuling, Zhu, Shuijin, Shi, Chunhai
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 29.03.2013; Public Library of Science (PLoS)
• Subjects: Agriculture; Amino acids; Amino Acids, Essential - genetics; Amino Acids, Essential - metabolism; Biology; Chromosome 15; Chromosome 5; Chromosomes; Cotton; Environmental effects; Environmental factors; Gene mapping; Gene-Environment Interaction; Genomes; Genotype & phenotype; Gossypium; Gossypium - genetics; Gossypium - metabolism; Gossypium hirsutum; Heredity; Inbreeding; Mapping; Oryza; Plants, Genetically Modified - genetics; Plants, Genetically Modified - metabolism; Quantitative Trait Loci; Seeds; Seeds - genetics; Seeds - metabolism; Studies; United Kingdom > UK; China
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0057531

Cottonseeds are rich in various essential amino acids. However, the inheritance of them at molecular level are still not defined across various genetic systems. In the present study, using a newly developed mapping model that can analyze the embryo and maternal main effects as well as QTL × environment interaction effects on quantitative quality trait loci (QTLs) in cottonseeds, a study on QTL located in the tetraploid embryo and tetraploid maternal plant genomes for essential amino acid contents in cottonseeds under different environments was carried out, using the immortal F2 (IF2) populations from a set of 188 recombinant inbred lines derived from an intraspecific hybrid cross of two upland cotton germplasms HS46 and MARKCBUCAG8US-1-88 as experimental materials. The results showed a total of 35 QTLs associated with these quality traits in cottonseeds. Nineteen QTLs were subsequently mapped on chromosome 5, 6 and 8 in sub-A genome and chromosome 15, 18, 22 and 23 in sub-D genome. Eighteen QTLs were also found having QTL × environment (QE) interaction effects. The genetic main effects from QTLs located on chromosomes in the embryo and maternal plant genomes and their QE effects in different environments were all important for these essential amino acids in cottonseeds. The results suggested that the influence of environmental factors on the expression of some QTLs located in different genetic systems should be considered when improving for these amino acids. This study can serve as the foundation for the improvement of these essential amino acids in cottonseeds.