Structure of cytotoxic associated antigen A protein of Helicobacter pylori from Bali and Lombok isolates of Indonesia

• Published in: Veterinary World Vol. 13; no. 7; pp. 1319 - 1326
• Main Authors: Suharsono, Hamong, Wibawa, Dewa Nyoman, Muttaqin, Zainul, Agustina, Kadek Karang
• Format: Journal Article
• Language: English
• Published: India Veterinary World 01.07.2020
• Subjects: Amino acids; Analysis; Antigens; bali; cytotoxic associated antigen a; Cytotoxicity; Genotypes; Helicobacter pylori; lombok; Phylogeny; Protein structure; Proteins; Statistical analysis; structure; Subculture; Variable region; Virulence; Virulence (Microbiology); East Asia; Indonesia; Bali; Lombok; Helicobacter pylori; virulence; cytotoxic associated antigen A; structure
• ISSN: 0972-8988; 2231-0916
• DOI: 10.14202/vetworld.2020.1319-1326

is a well-known zoonotic agent with worldwide distribution. In Indonesia, only one report regarding the variation within the cytotoxic associated antigen A (CagA) protein of has been described in the literature, which was conducted in Manado, South Sulawesi. There remains no report concerning the structure of this protein, particularly for the Bali and Lombok isolates. The objective of this study was to investigate the diversity o i CagA amino acid sequences of Bali and Lombok isolates, to predict their molecular structures and conduct toxicity examination of CagA on gastric cells. A total of 36 samples were used in equal proportions for each pathologic condition. DNA extraction was performed to subculture Bali isolates. The amplification of the CagA 3' variable region was carried out using the primers P1 (5'-GATAACAGGCAAGCTTTTTGAGG-3') and P2 (5'-CTGCAAAAGATTGTTTGGCAG-3'). The W2, W9, and W35 fragments were selected as a representation of Bali isolates, which were modeled through the threading modeling approach using I-TASSER. According to the 12 CagA sequences obtained and phylogenetic analyses, the strain originating from Bali can be grouped within the East Asian genotypes and is identical to the Lombok strain. In addition, the Bali isolates are phylogenetically more closely related to Southeast Asian strains, particularly the Filipino strain. The relationship between degree of inflammation induced and CagA-positive infection was not statistically significant. The structure of the Bali isolate is identical to that of Lombok isolate, which belongs to the same group of East Asian genotypes, and bacterial virulence is not related to structure.