Effect of somatic growth, strain, and sex on double-chamber plethysmographic respiratory function values in healthy mice
Departments of 1 Pathology and 2 Biostatistics, University of Liège, B-4000 Liège, Belgium Double-chamber plethysmography has been recognized since 1979 as a reference technique to measure pulmonary function values in guinea pigs, but it has not gained attention for use in mice. Theoretically, how...
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Published in | Journal of applied physiology (1985) Vol. 94; no. 3; pp. 1129 - 1136 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
Bethesda, MD
Am Physiological Soc
01.03.2003
American Physiological Society |
Subjects | |
Online Access | Get full text |
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Summary: | Departments of 1 Pathology and
2 Biostatistics, University of Liège, B-4000
Liège, Belgium
Double-chamber plethysmography
has been recognized since 1979 as a reference technique to
measure pulmonary function values in guinea pigs, but it has not gained
attention for use in mice. Theoretically, however, this technique
combines the advantages of single-chamber plethysmography with a
quantitative assessment of flow and/or volume and a calculated
resistance, the interpretation of which in terms of
bronchoconstriction is not disputed. Here we show that, when
appropriately preconditioned, mice are able to gradually grow
accustomed to the apparatus and display extremely stable nasal and
thoracoabdominal flow tracings. Overall, strain, sex, and somatic
growth had a significant effect on pulmonary function values. The
changes in specific airway resistance (sRaw) and enhanced pause (Penh)
values were never in the same direction, indicating that they measure
different things. The respiratory frequency was far higher in C57BL/6
compared with BALB/c mice. Peak flows, minute volume, specific tidal
and minute volumes, and sRaw were also higher, but Penh was smaller.
Males breathed at a higher frequency than females, leading to a higher
minute volume. Nevertheless, the specific volumes were considerably
higher among females. Penh was lower in males, whereas sRaw was
identical in both sexes. Changes associated with somatic growth were
rapid and important between 5 and 9 wk, then slowed down between 9 and 12-13 wk and became almost imperceptible after.
airway resistance; BALB/c; C57BL/6 |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 8750-7587 1522-1601 |
DOI: | 10.1152/japplphysiol.00561.2002 |