Tracking the dispersion of Scaphoideus titanus Ball (Hemiptera: Cicadellidae) from wild to cultivated grapevine: use of a novel mark–capture technique

• Published in: Bulletin of entomological research Vol. 104; no. 4; pp. 432 - 443
• Main Authors: Lessio, F., Tota, F., Alma, A.
• Format: Journal Article
• Language: English
• Published: Cambridge, UK Cambridge University Press 01.08.2014
• Subjects: adults; Albumins; Animal Distribution - physiology; Animals; Biological and medical sciences; biological corridors; Biomarkers; Biomarkers - metabolism; canopy; casein; Caseins; chickens; Cicadellidae; computer software; Cultivation; Dispersion; Ecosystem; egg albumen; eggs; Enzyme-Linked Immunosorbent Assay; Female; Flowers & plants; Fruit cultivation; Fundamental and applied biological sciences. Psychology; Geographic Information Systems; grapes; Hemiptera; Hemiptera - physiology; Insects; Male; males; Milk; Pest control; pest management; Phytopathology. Animal pests. Plant and forest protection; Protozoa. Invertebrates; rain; Records, symptoms, damages, economic importance, population surveys; Regression Analysis; Research Paper; Scaphoideus titanus; seeds; Specimen Handling - methods; sticky traps; Vineyards; Vitaceae; Vitis; Vitis - parasitology; Wineries & vineyards; dispersal; immunomarking; leafhopper vector; spatial interpolation; ELISA; Tracking; Plant pathogen; Insecta; Scaphoideus titanus; Dispersion; Population survey; Cicadomorpha; Pest; Homoptera; Capture recapture method; Fruit crop; Arthropoda; Wild plant; Population dynamics; Cultivated plant; Invertebrata; Cicadellidae; Vector
• ISSN: 0007-4853; 1475-2670; 1475-2670
• DOI: 10.1017/S0007485314000030

The dispersion of Scaphoideus titanus Ball adults from wild to cultivated grapevines was studied using a novel mark–capture technique. The crowns of wild grapevines located at a distance from vineyards ranging from 5 to 330 m were sprayed with a water solution of either cow milk (marker: casein) or chicken egg whites (marker: albumin) and insects captured in yellow sticky traps placed on the canopy of grapes were analyzed via an indirect ELISA for markers’ identification. Data were subject to exponential regression as a function of distance from wild grapevine, and to spatial interpolation (Inverse Distance Weighted and Kernel interpolation with barriers) using ArcGIS Desktop 10.1 software. The influence of rainfall and time elapsed after marking on markers’ effectiveness, and the different dispersion of males and females were studied with regression analyses. Of a total of 5417 insects analyzed, 43% were positive to egg; whereas 18% of 536 tested resulted marked with milk. No influence of rainfall or time elapsed was observed for egg, whereas milk was affected by time. Males and females showed no difference in dispersal. Marked adults decreased exponentially along with distance from wild grapevine and up to 80% of them were captured within 30 m. However, there was evidence of long-range dispersal up to 330 m. The interpolation maps showed a clear clustering of marked S. titanus close to the treated wild grapevine, and the pathways to the vineyards did not always seem to go along straight lines but mainly along ecological corridors. S. titanus adults are therefore capable of dispersing from wild to cultivated grapevine, and this may affect pest management strategies.