Autophagy induced by HIF1α overexpression supports trophoblast invasion by supplying cellular energy

• Published in: PloS one Vol. 8; no. 10; p. e76605
• Main Authors: Yamanaka-Tatematsu, Mikiko, Nakashima, Akitoshi, Fujita, Naonobu, Shima, Tomoko, Yoshimori, Tamotsu, Saito, Shigeru
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 03.10.2013; Public Library of Science (PLoS)
• Subjects: Adenosine triphosphate; Adenosine Triphosphate - metabolism; ATP; Autophagy; Autophagy - genetics; Cell Adhesion; Cell death; Cell Line; Cell Movement; Cobalt; Cobalt - pharmacology; Cobalt chloride; Decidua; Decidua - cytology; Decidua - metabolism; Dietary supplements; Energy consumption; Energy Metabolism; Female; Gelatinase B; Gene Expression Regulation, Developmental; Gynecology; Homeostasis; Humans; Hypoxia; Hypoxia - metabolism; Hypoxia-Inducible Factor 1, alpha Subunit - agonists; Hypoxia-Inducible Factor 1, alpha Subunit - genetics; Hypoxia-Inducible Factor 1, alpha Subunit - metabolism; Invasiveness; Ion channels; Matrix Metalloproteinase 9 - genetics; Matrix Metalloproteinase 9 - metabolism; Medicine; Nutrition; Obstetrics; Oxygen; Penicillin; Peptides; Phagocytosis; Physiology; Placenta; Preeclampsia; Pregnancy; Pregnancy Trimester, First; Purine P2X receptors; Quantitative analysis; Receptors, Purinergic P2X7 - genetics; Receptors, Purinergic P2X7 - metabolism; Rodents; Signal Transduction; Transcription factors; Trophoblasts; Trophoblasts - cytology; Trophoblasts - metabolism; Veins & arteries; United States > US; Japan
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0076605

Extravillous trophoblasts (EVTs) characterize the invasion of the maternal decidua under low oxygen and poor nutrition at the early feto-maternal interface to establish a successful pregnancy. We previously reported that autophagy in EVTs was activated under 2% O2 in vitro, and autophagy activation was also observed in EVTs at the early feto-maternal interface in vivo. Here, we show that autophagy is an energy source for the invasion of EVTs. Cobalt chloride (CoCl2), which induces hypoxia inducible factor 1α (HIF1α) overexpression, activated autophagy in HTR8/SVneo cells, an EVT cell line. The number of invading HTR8-ATG4B(C74A) cells, an autophagy-deficient EVT cell line, was markedly reduced by 81 percent with the CoCl2 treatment through the suppression of MMP9 level, although CoCl2 did not affect the cellular invasion of HTR8-mStrawberry cells, a control cell line. HTR8-ATG4B(C74A) cells treated with CoCl2 showed a decrease in cellular adenosine triphosphate (ATP) levels and a compensatory increase in the expression of purinergic receptor P2X ligand-gated ion channel 7 (P2RX7), which is stimulated with ATP, whereas HTR8-mStrawberry cells maintained cellular ATP levels and did not affect P2RX7 expression. Furthermore, the decreased invasiveness of HTR8-ATG4B(C74A) cells treated with CoCl2 was neutralized by ATP supplementation to the level of HTR8-ATG4B(C74A) cells treated without CoCl2. These results suggest that autophagy plays a role in maintaining homeostasis by countervailing HIF1α-mediated cellular energy consumption in EVTs.