Partitioning the efficiency of utilization of amino acids in growing broilers: Multiple linear regression and multivariate approaches

• Published in: PloS one Vol. 13; no. 12; p. e0208488
• Main Authors: Reis, Matheus De Paula, Sakomura, Nilva Kazue, Teixeira, Izabelle A M A, da Silva, Edney Pereira, Kebreab, Ermias
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 12.12.2018; Public Library of Science (PLoS)
• Subjects: Age factors; Amino acids; Amino Acids - metabolism; Amino Acids, Sulfur - metabolism; Animal Feed - analysis; Animal Feed - statistics & numerical data; Animal Nutritional Physiological Phenomena; Animal sciences; Animals; Biology and Life Sciences; Body Composition - drug effects; Body Composition - physiology; Chickens; Chickens - growth & development; Chickens - metabolism; Deposition; Diet; Diet - statistics & numerical data; Dietary Proteins - pharmacokinetics; Earth Sciences; Ecology and Environmental Sciences; Efficiency; Energy Metabolism - physiology; Feathers; Feeds; Hogs; Linear Models; Lysine; Lysine - metabolism; Male; Medicine and Health Sciences; Multivariate Analysis; Nitrogen; Nutrition research; Partitioning; Physical Sciences; Poultry; Predictions; Proteins; Regression analysis; Research and Analysis Methods; Studies; Sulfur; Threonine; Threonine - metabolism; Utilization; Valine; Valine - metabolism; Weight Gain - physiology; Zoology; Brazil; United States > US; California
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0208488

Determining the efficiency of amino acid (AA) utilization in growing animals is crucial to estimate their requirement accurately. In broiler chickens, the composition of AA in feather is different from feather-free body and the proportion of feathers will change along broiler's growth, which may impact the efficiency of utilization on AA consumed. Therefore, in order to establish a method that predicts the efficiency of utilization for feather-free body and feather, two approaches were evaluated: a multiple linear regression and a multivariate analysis. Additionally, a new factorial model was proposed to predict AA requirements in broiler chickens. Data from 13 trials that evaluated the requirements for lysine (Lys), sulphur AA (SAA), threonine (Thr), and valine (Val) in male broilers were used for the analyses. Both methods of analysis were consistent in showing that the efficiency of utilization in feather-free body and feather were different. Using multiple linear regression, the values of efficiency of utilization estimated in feather-free body were 0.68, 0.72, 0.81, 0.79 (mg of amino acid deposited / mg of amino acid consumed above maintenance) and in feather were 0.58, 0.77, 0.78, and 1.57 (mg/mg) for Lys, SAA, Thr, and Val, respectively. Applying the multivariate approach, the corresponding predicted values were 0.68, 0.67, 4.23, 0.27 (mg/mg) in feather-free body and 1.16, 0.86, 0.16, and 1.10 (mg/mg) in feather, respectively. According to the results, efficiency of utilization may be related, to some extent, on the concentration determined in each tissue. The uncertainty around the amount of AA consumed for gain directed to feather-free body or feather deposition could be a limitation for multivariate analyses. The results indicated that multiple linear regression predictions may be better estimates of utilization efficiency. However, more studies are needed to elucidate the effect of age on deposition and partitioning of dietary AA in different parts of the broiler.