Identification of novel miRNAs and miRNA expression profiling in wheat hybrid necrosis

• Published in: PloS one Vol. 10; no. 2; p. e0117507
• Main Authors: Zhou, Jianping, Cheng, Yan, Yin, Meiqi, Yang, Ennian, Gong, Wenping, Liu, Cheng, Zheng, Xuelian, Deng, Kejun, Ren, Zhenglong, Zhang, Yong
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 23.02.2015; Public Library of Science (PLoS)
• Subjects: Arabidopsis; Arabidopsis thaliana; Barley; Biological activity; Environmental changes; Flowers & plants; Gangrene; Gene expression; Gene Expression Profiling; Genomics; Life sciences; MicroRNAs; MicroRNAs - genetics; miRNA; Molecular chains; Necrosis; Nicotiana tabacum; Oryza sativa; Plant Diseases - genetics; Proteins; Ribonucleic acid; RNA; RNA polymerase; Triticum - genetics; Triticum aestivum; Wheat; China
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0117507

MicroRNAs (miRNAs) play essential roles in a vast array of biological processes, including growth and development, defense against viral infection, and responses to environmental changes in plant. Wheat hybrid necrosis is an interesting genetic phenomenon observed frequency and it is lethal or semi lethal, resulting in gradual death or loss of productivity. However, the molecular basis and mechanisms associated with hybrid necrosis in wheat are still not well understood. Here, we report the population and expression profiles of miRNAs in wheat hybrid necrosis. We identified a total of 57 conserved miRNA families as well as 182 putative novel miRNAs. Expression profiling revealed that expression of 49 known miRNAs and 165 novel miRNAs was changed in hybrid necrosis. And the expression levels of some miRNAs and their predicated targets have been confirmed by qRT-PCR. These results indicate that these miRNAs, especially miR159, miR166, miR167 and miR5072 could be involved in the extensive regulation of gene expression in response to hybrid necrosis.