Cellular membrane cholesterol is required for porcine reproductive and respiratory syndrome virus entry and release in MARC-145 cells

• Published in: Science China. Life sciences Vol. 54; no. 11; pp. 1011 - 1018
• Main Authors: Sun, Ying, Xiao, ShaoBo, Wang, Dang, Luo, Rui, Li, Bin, Chen, HuanChun, Fang, LiuRong
• Format: Journal Article
• Language: English
• Published: Beijing Science China Press 01.11.2011; Springer Nature B.V
• Subjects: Animals; beta-Cyclodextrins - pharmacology; Biomedical and Life Sciences; Cell Line; Cell Membrane - chemistry; Cell Membrane - drug effects; Cell Membrane - metabolism; Cell Survival; Cholesterol - metabolism; Life Sciences; Porcine Reproductive and Respiratory Syndrome - metabolism; Porcine respiratory and reproductive syndrome virus; Porcine respiratory and reproductive syndrome virus - genetics; Porcine respiratory and reproductive syndrome virus - metabolism; Porcine respiratory and reproductive syndrome virus - pathogenicity; PRRSV感染; Research Papers; Swine; Virus Internalization; Virus Release; 入境; 抑制作用; 猪繁殖与呼吸综合征病毒; 环糊精; 组成部分; 细胞膜; 胆固醇; virus entry; cholesterol; release; porcine reproductive and respiratory syndrome virus (PRRSV); membrane fusion
• ISSN: 1674-7305; 1869-1889
• DOI: 10.1007/s11427-011-4236-0

Cholesterol represents one of the key constituents of small,dynamic,sterol-and sphingolipid-enriched domains on the plasma membrane.It has been reported that many viruses depend on plasma membrane cholesterol for efficient infection.In this study,the role of the plasma membrane cholesterol in porcine reproductive and respiratory syndrome virus（PRRSV） infection of MARC-145 cells was investigated.Pretreatment of MARC-145 cells with methyl-β-cyclodextrin（MβCD）,a drug used to deplete cholesterol from cellular membrane,significantly reduced PRRSV infection in a dose-dependent manner.This inhibition was partially reversed by supplementing exogenous cholesterol following MβCD treatment,suggesting that the inhibition of PRRSV infection was specifically mediated by removal of cellular cholesterol.Further detailed studies showed that depletion of cellular membrane cholesterol significantly inhibited virus entry,especially virus attachment and release.These results indicate that the presence of cholesterol in the cellular membrane is a key component of PRRSV infection.