Disruption of SCO5461 Gene Coding for a Mono-ADP-Ribosyltransferase Enzyme Produces a Conditional Pleiotropic Phenotype Affecting Morphological Differentiation and Antibiotic Production in Streptomyces coelicolor

• Published in: The journal of microbiology Vol. 50; no. 3; pp. 409 - 418
• Main Authors: Szirak, Krisztina, University of Debrecen, Debrecen, Hungary, Keseru, Judit, University of Debrecen, Debrecen, Hungary, Biro, Sandor, University of Debrecen, Debrecen, Hungary, Schmelczer, Ivan, University of Debrecen, Debrecen, Hungary, Barabas, Gyoergy, University of Debrecen, Debrecen, Hungary, Penyige, Andras, University of Debrecen, Debrecen, Hungary
• Format: Journal Article
• Language: English
• Published: Heidelberg The Microbiological Society of Korea 01.06.2012; Springer Nature B.V; 한국미생물학회
• Subjects: actinorhodin production and secretion; Adenosine diphosphate; ADP Ribose Transferases - deficiency; ADP-ribosylation; Anti-Bacterial Agents - biosynthesis; Antibiotics; Biomedical and Life Sciences; Cholera; Culture Media - chemistry; Differentiation; E coli; Enzymes; Gene Deletion; Genes; Genotype & phenotype; Life Sciences; Membrane proteins; Microbiology; mono-ADP-ribosyltransferase; morphological differentiation; Morphology; Mutants; NAD(P) super(+)-arginine ADP-ribosyltransferase; Osmotic Pressure; Plasmids; protein ADP-ribosylation; Protein Processing, Post-Translational; Proteins; Proteome - analysis; Regulation; Secretion; Spores, Bacterial - cytology; STREPTOMYCES; Streptomyces coelicolor; Streptomyces coelicolor - cytology; Streptomyces coelicolor - enzymology; Streptomyces coelicolor - genetics; Streptomyces coelicolor - metabolism; 생물학; mono-ADP-ribosyltransferase; protein ADP-ribosylation; morphological differentiation; actinorhodin production and secretion
• ISSN: 1225-8873; 1976-3794
• DOI: 10.1007/s12275-012-1440-y

The SCO5461 gene of Streptomyces coelicolor A3(2) codes for an ADP-ribosyltransferase enzyme that is predicted to be a transmembrane protein with an extracellular catalytic domain. PCR-targeted disruption of the gene resulted in a mutant that differentiated normally on complex SFM medium; however, morphological differentiation in minimal medium was significantly delayed and this phenotype was even more pronounced on osmotically enhanced minimal medium. The mutant did not sporulate when it was grown on R5 medium, however the normal morphological differentiation was restored when the strain was cultivated beside the wild-type S. coelicolor M145 strain. Comparison of the pattern of ADP-ribosylated proteins showed a difference between the mutant and the wild type, fewer modified proteins were present in the cellular crude extract of the mutant strain. These results support our previous suggestions that protein ADP-ribosylation is involved in the regulation of differentiation and antibiotic production and secretion in Streptomyces.