Proteome Analysis of Poplar Seed Vigor

• Published in: PloS one Vol. 10; no. 7; p. e0132509
• Main Authors: Zhang, Hong, Wang, Wei-Qing, Liu, Shu-Jun, Møller, Ian Max, Song, Song-Quan
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 14.07.2015; Public Library of Science (PLoS)
• Subjects: Abundance; Aging; Botany; Cotton; Electrolyte leakage; Energy; Energy storage; Germination; Heat; Heat shock proteins; Humidity; Laboratories; Metabolism; Plant Proteins - metabolism; Plant sciences; Poplar; Populus - growth & development; Populus - metabolism; Populus - physiology; Protein biosynthesis; Protein folding; Protein synthesis; Protein turnover; Proteins; Proteomes; Proteomics; Relative humidity; Seed germination; Seeds; Seeds - growth & development; Seeds - metabolism; Seeds - physiology; Spots; Storage proteins; Temperature; Temperature range; Time Factors; Transcriptome; Vigor
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0132509

Seed vigor is a complex property that determines the seed's potential for rapid uniform emergence and subsequent growth. However, the mechanism for change in seed vigor is poorly understood. The seeds of poplar (Populus × Canadensis Moench), which are short-lived, were stored at 30 °C and 75 ± 5% relative humidity for different periods of time (0-90 days) to obtain different vigor seeds (from 95 to 0% germination). With decreasing seed vigor, the temperature range of seed germination became narrower; the respiration rate of the seeds decreased markedly, while the relative electrolyte leakage increased markedly, both levelling off after 45 days. A total of 81 protein spots showed a significant change in abundance (≥ 1.5-fold, P < 0.05) when comparing the proteomes among seeds with different vigor. Of the identified 65 proteins, most belonged to the groups involved in metabolism (23%), protein synthesis and destination (22%), energy (18%), cell defense and rescue (17%), and storage protein (15%). These proteins accounted for 95% of all the identified proteins. During seed aging, 53 and 6 identified proteins consistently increased and decreased in abundance, respectively, and they were associated with metabolism (22%), protein synthesis and destination (22%), energy (19%), cell defense and rescue (19%), storage proteins (15%), and cell growth and structure (3%). These data show that the decrease in seed vigor (aging) is an energy-dependent process, which requires protein synthesis and degradation as well as cellular defense and rescue.