Ultraviolet Light (UV) Inactivation of Porcine Parvovirus in Liquid Plasma and Effect of UV Irradiated Spray Dried Porcine Plasma on Performance of Weaned Pigs
A novel ultraviolet light irradiation (UV-C, 254 nm) process was designed as an additional safety feature for manufacturing of spray dried porcine plasma (SDPP). In Exp. 1, three 10-L batches of bovine plasma were inoculated with 10(5.2 ± 0.12) tissue culture infectious dose 50 (TCID50) of porcine p...
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| Published in | PloS one Vol. 10; no. 7; p. e0133008 |
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| Main Authors | , , , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
United States
Public Library of Science
14.07.2015
Public Library of Science (PLoS) |
| Subjects | |
| Online Access | Get full text |
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| Summary: | A novel ultraviolet light irradiation (UV-C, 254 nm) process was designed as an additional safety feature for manufacturing of spray dried porcine plasma (SDPP). In Exp. 1, three 10-L batches of bovine plasma were inoculated with 10(5.2 ± 0.12) tissue culture infectious dose 50 (TCID50) of porcine parvovirus (PPV) per mL of plasma and subjected to UV-C ranging from 0 to 9180 J/L. No viable PPV was detected in bovine plasma by micro-titer assay in SK6 cell culture after UV-C at 2295 J/L. In Exp. 2, porcine plasma was subjected to UV-C (3672 J/L), then spray dried and mixed in complete mash diets. Diets were a control without SDPP (Control), UV-C SDPP either at 3% (UVSDPP3) or 6% (UVSDPP6) and non-UV-C SDPP at 3% (SDPP3) or 6% (SDPP6). Diets were fed ad libitum to 320 weaned pigs (26 d of age; 16 pens/diet; 4 pigs/pen) for 14 d after weaning and a common diet was fed d 15 to 28. During d 0 to 14, pigs fed UVSDPP3, UVSDPP6, or SDPP6 had higher (P < 0.05) weight gain and feed intake than control. During d 0 to 28, pigs fed UVSDPP3 and UVSDPP6 had higher (P < 0.05) weight gain and feed intake than control and SDPP3, and SDPP6 had higher (P < 0.05) feed intake than control. Also, pigs fed UVSDPP had higher (P < 0.05) weight gain than pigs fed SDPP. In conclusion, UV-C inactivated PPV in liquid plasma and UVSDPP used in pig feed had no detrimental effects on pig performance. |
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| Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Competing Interests: The authors have read the journal's policy and the authors of this manuscript have the following competing interests: JP, CR and JR are employed by APC Europe, S.A., Granollers, Spain. JDC, JMC, and LER are employed by APC Inc., Ankeny, IA, whose companies partly funded this study. Both, APC Europe, S.A. and APC Inc., manufactures and sells spray dried plasma. Joan Pujols, and David Torrallardona declare no conflict of interest. APC Inc and AP Europe, S.A. manufacture the following spray dried plasma products for swine use: AP820, AP820P, AP820B, AP920, AP920P, AP920B, Appetein (US Patent N° 6004576) and Appetein GS. However, any of these commercial products were used in this study because the spray dried plasma used in experiment 2 was produced at pilot plant level, as it is indicated in the manuscript. This does not alter the authors' adherence to all the PLOS ONE policies on sharing data and materials. Conceived and designed the experiments: J. Polo CR JR LER JMC JDC DT J. Pujols. Performed the experiments: J. Polo CR JR DT J. Pujols. Analyzed the data: J. Polo LER JMC JDC DT J. Pujols. Contributed reagents/materials/analysis tools: J. Polo CR JR DT J. Pujols. Wrote the paper: J. Polo CR JR LER JMC JDC DT J. Pujols. |
| ISSN: | 1932-6203 1932-6203 |
| DOI: | 10.1371/journal.pone.0133008 |