Structural Flexibility in the Burkholderia mallei Genome

The complete genome sequence of Burkholderia mallei ATCC23344 provides insight into this highly infectious bacterium's pathogenicity and evolutionary history. B. mallei, the etiologic agent of glanders, has come under renewed scientific investigation as a result of recent concerns about its pas...

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Published inProceedings of the National Academy of Sciences - PNAS Vol. 101; no. 39; pp. 14246 - 14251
Main Authors Nierman, William C., DeShazer, David, Kim, H. Stanley, Tettelin, Herve, Nelson, Karen E., Feldblyum, Tamara, Ulrich, Ricky L., Ronning, Catherine M., Brinkac, Lauren M., Daugherty, Sean C., Davidsen, Tanja D., Deboy, Robert T., Dimitrov, George, Dodson, Robert J., Durkin, A. Scott, Gwinn, Michelle L., Haft, Daniel H., Khouri, Hoda, Kolonay, James F., Madupu, Ramana, Mohammoud, Yasmin, Nelson, William C., Radune, Diana, Romero, Claudia M., Sarria, Saul, Selengut, Jeremy, Shamblin, Christine, Sullivan, Steven A., White, Owen, Yu, Yan, Zafar, Nikhat, Zhou, Liwei, Fraser, Claire M., Greenberg, E. Peter
Format Journal Article
LanguageEnglish
Published United States National Academy of Sciences 28.09.2004
National Acad Sciences
SeriesFrom the Cover
Subjects
Animals
Bacteria
Base Composition - genetics
Base Sequence
Biological Sciences
Burkholderia
Burkholderia mallei - genetics
Burkholderia mallei - pathogenicity
Chromosomes
Chromosomes, Bacterial - genetics
Cricetinae
Evolutionary biology
Genes
Genetic variation
Genetics
Genome, Bacterial
Genomes
Genomics
Glanders - microbiology
Immunology
Liver
Liver - metabolism
Mesocricetus
Microbiology
Molecular Sequence Data
Multigene Family
Oligonucleotide Array Sequence Analysis
Online searching
Open reading frames
Open Reading Frames - genetics
Virulence
Online AccessGet full text

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Summary:The complete genome sequence of Burkholderia mallei ATCC23344 provides insight into this highly infectious bacterium's pathogenicity and evolutionary history. B. mallei, the etiologic agent of glanders, has come under renewed scientific investigation as a result of recent concerns about its past and potential future use as a biological weapon. Genome analysis identified a number of putative virulence factors whose function was supported by comparative genome hybridization and expression profiling of the bacterium in hamster liver in vivo. The genome contains numerous insertion sequence elements that have mediated extensive deletions and rearrangements of the genome relative to Burkholderia pseudomallei. The genome also contains a vast number (>12,000) of simple sequence repeats. Variation in simple sequence repeats in key genes can provide a mechanism for generating antigenic variation that may account for the mammalian host's inability to mount a durable adaptive immune response to a B. mallei infection.
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Abbreviations: CDS, coding sequence; TIGR, The Institute for Genomic Research; cfu, colony-forming units; IS, insertion sequence; SSR, simple sequence repeat.
Data deposition: The sequences reported in this paper have been deposited in the GenBank database (accession nos. CP000010 and CP000011).
To whom correspondence should be addressed. E-mail: wnierman@tigr.org.
This paper was submitted directly (Track II) to the PNAS office.
Edited by E. Peter Greenberg, University of Iowa, Iowa City, IA, and approved July 26, 2004
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.0403306101