Detection of Osteogenic Differentiation by Differential Mineralized Matrix Production in Mesenchymal Stromal Cells by Raman Spectroscopy

• Published in: PloS one Vol. 8; no. 5; p. e65438
• Main Authors: Hung, Pei-San, Kuo, Yi-Chun, Chen, He-Guei, Chiang, Hui-Hua Kenny, Lee, Oscar Kuang-Sheng
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 29.05.2013; Public Library of Science (PLoS)
• Subjects: Biocompatibility; Biology; Biosensors; Bone marrow; Calcium Phosphates - metabolism; Cell Adhesion Molecules - genetics; Cell Differentiation; Cells, Cultured; Collagen Type I - genetics; Core Binding Factor Alpha 1 Subunit - genetics; Differentiation; Durapatite - metabolism; Fibroblasts; Gene Expression Profiling; Glycerol; Growth factors; Humans; Hydroxyapatite; Lysis; Maturation; Medicine; Mesenchymal stem cells; Mesenchymal Stromal Cells - cytology; Mesenchymal Stromal Cells - metabolism; Mesenchyme; Mineralization; Molecular biology; Osteoblastogenesis; Osteogenesis; Physics; Polymerase chain reaction; R&D; Raman spectroscopy; Real time; Regenerative medicine; Reproducibility of Results; Research & development; Reverse Transcriptase Polymerase Chain Reaction; Selectivity; Spectroscopy; Spectrum analysis; Spectrum Analysis, Raman - methods; Stem cells; Stromal cells; Studies; Therapeutic applications; Tissue engineering; Tissue Engineering - methods; Taiwan
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0065438

Mesenchymal stromal cells (MSCs) hold great potential in skeletal tissue engineering and regenerative medicine. However, conventional methods that are used in molecular biology to evaluate osteogenic differentiation of MSCs require a relatively large amount of cells. Cell lysis and cell fixation are also required and all these steps are time-consuming. Therefore, it is imperative to develop a facile technique which can provide real-time information with high sensitivity and selectivity to detect the osteogenic maturation of MSCs. In this study, we use Raman spectroscopy as a biosensor to monitor the production of mineralized matrices during osteogenic induction of MSCs. In summary, Raman spectroscopy is an excellent biosensor to detect the extent of maturation level during MSCs-osteoblast differentiation with a non-disruptive, real-time and label free manner. We expect that this study will promote further investigation of stem cell research and clinical applications.