Effect of apoA-I Mutations in the Capacity of Reconstituted HDL to Promote ABCG1-Mediated Cholesterol Efflux

• Published in: PloS one Vol. 8; no. 6; p. e67993
• Main Authors: Daniil, Georgios, Zannis, Vassilis I, Chroni, Angeliki
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 27.06.2013; Public Library of Science (PLoS)
• Subjects: ABCA1 protein; Animals; Apolipoprotein A; Apolipoprotein A-I; Apolipoprotein A-I - genetics; Apolipoprotein A-I - isolation & purification; Apolipoprotein A-I - metabolism; Apolipoproteins; ATP Binding Cassette Transporter, Subfamily G, Member 1 - metabolism; ATP-binding protein; Biochemistry; Biology; Cardiovascular disease; Cell Membrane - metabolism; Cellular structure; Cholesterol; Cholesterol, HDL - metabolism; Clonal deletion; Deletion mutant; Efflux; HEK293 Cells; Helices; High density lipoprotein; Humans; Ketocholesterols - metabolism; Lipids; Lipoproteins; Localization; Macrophages; Medicine; Mice; Mutants; Mutation; Protein Domains; Proteins; Proteolysis; Recombinant Proteins - genetics; Recombinant Proteins - isolation & purification; Recombinant Proteins - metabolism; Rodents; Sterols; Structure-Activity Relationship; Structure-function relationships; Studies; United States > US
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0067993

ATP binding cassette transporter G1 (ABCG1) mediates the cholesterol transport from cells to high-density lipoprotein (HDL), but the role of apolipoprotein A-I (apoA-I), the main protein constituent of HDL, in this process is not clear. To address this, we measured cholesterol efflux from HEK293 cells or J774 mouse macrophages overexpressing ABCG1 using as acceptors reconstituted HDL (rHDL) containing wild-type or various mutant apoA-I forms. It was found that ABCG1-mediated cholesterol efflux was severely reduced (by 89%) when using rHDL containing the carboxyl-terminal deletion mutant apoA-I[Δ(185-243)]. ABCG1-mediated cholesterol efflux was not affected or moderately decreased by rHDL containing amino-terminal deletion mutants and several mid-region deletion or point apoA-I mutants, and was restored to 69-99% of control by double deletion mutants apoA-I[Δ(1-41)Δ(185-243)] and apoA-I[Δ(1-59)Δ(185-243)]. These findings suggest that the central helices alone of apoA-I associated to rHDL can promote ABCG1-mediated cholesterol efflux. Further analysis showed that rHDL containing the carboxyl-terminal deletion mutant apoA-I[Δ(185-243)] only slightly reduced (by 22%) the ABCG1-mediated efflux of 7-ketocholesterol, indicating that depending on the sterol type, structural changes in rHDL-associated apoA-I affect differently the ABCG1-mediated efflux of cholesterol and 7-ketocholesterol. Overall, our findings demonstrate that rHDL-associated apoA-I structural changes affect the capacity of rHDL to accept cellular cholesterol by an ABCG1-mediated process. The structure-function relationship seen here between rHDL-associated apoA-I mutants and ABCG1-mediated cholesterol efflux closely resembles that seen before in lipid-free apoA-I mutants and ABCA1-dependent cholesterol efflux, suggesting that both processes depend on the same structural determinants of apoA-I.