Expression Cloning of Gamma Interferon-Inducing Antigens of Mycobacterium avium subsp. paratuberculosis

• Published in: Infection and Immunity Vol. 73; no. 6; pp. 3778 - 3782
• Main Authors: Nagata, Reiko, Muneta, Yoshihiro, Yoshihara, Kazuhiro, Yokomizo, Yuichi, Mori, Yasuyuki
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for Microbiology 01.06.2005
• Subjects: Amino Acid Motifs; Animals; Antigens, Bacterial - chemistry; Antigens, Bacterial - genetics; Antigens, Bacterial - immunology; Biological and medical sciences; Cattle; Cellular Microbiology: Pathogen-Host Cell Molecular Interactions; Cloning, Molecular; Enzyme-Linked Immunosorbent Assay; Escherichia coli; Fundamental and applied biological sciences. Psychology; Interferon-gamma - biosynthesis; Microbiology; Mycobacterium avium; Mycobacterium avium subsp. paratuberculosis - immunology; Recombinant Proteins - biosynthesis; Recombinant Proteins - chemistry; Recombinant Proteins - immunology; Microbiology; Paratuberculosis; Cytokine; Mycobacterium avium; Mycobacterial infection; Immunity; Infection; Antigen; Mycobacteriales; Bacteriosis; Mycobacteriaceae; Bacteria; Actinomycetes; Gamma interferon

Three recombinant proteins, Map10, Map39, and Map41, produced based on nucleotide sequences obtained from the screening of Mycobacterium avium subsp. paratuberculosis genomic library expressed in Escherichia coli significantly elicited gamma interferon production in peripheral blood mononuclear cell...