Expression Cloning of Gamma Interferon-Inducing Antigens of Mycobacterium avium subsp. paratuberculosis

Three recombinant proteins, Map10, Map39, and Map41, produced based on nucleotide sequences obtained from the screening of Mycobacterium avium subsp. paratuberculosis genomic library expressed in Escherichia coli significantly elicited gamma interferon production in peripheral blood mononuclear cell...

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Bibliographic Details
Published inInfection and Immunity Vol. 73; no. 6; pp. 3778 - 3782
Main Authors Nagata, Reiko, Muneta, Yoshihiro, Yoshihara, Kazuhiro, Yokomizo, Yuichi, Mori, Yasuyuki
Format Journal Article
LanguageEnglish
Published Washington, DC American Society for Microbiology 01.06.2005
Subjects
Amino Acid Motifs
Animals
Antigens, Bacterial - chemistry
Antigens, Bacterial - genetics
Antigens, Bacterial - immunology
Biological and medical sciences
Cattle
Cellular Microbiology: Pathogen-Host Cell Molecular Interactions
Cloning, Molecular
Enzyme-Linked Immunosorbent Assay
Escherichia coli
Fundamental and applied biological sciences. Psychology
Interferon-gamma - biosynthesis
Microbiology
Mycobacterium avium
Mycobacterium avium subsp. paratuberculosis - immunology
Recombinant Proteins - biosynthesis
Recombinant Proteins - chemistry
Recombinant Proteins - immunology
Microbiology
Paratuberculosis
Cytokine
Mycobacterium avium
Mycobacterial infection
Immunity
Infection
Antigen
Mycobacteriales
Bacteriosis
Mycobacteriaceae
Bacteria
Actinomycetes
Gamma interferon
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Summary:Three recombinant proteins, Map10, Map39, and Map41, produced based on nucleotide sequences obtained from the screening of Mycobacterium avium subsp. paratuberculosis genomic library expressed in Escherichia coli significantly elicited gamma interferon production in peripheral blood mononuclear cells from infected cattle. Two of these proteins were members of the PPE protein family.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Corresponding author. Mailing address: Immune System Section, Department of Immunology, National Institute of Animal Health, 3-1-5 Kannondai, Tsukuba, Ibaraki, Japan 305-0856. Phone: 81-29-838-7857. Fax: 81-29-838-7880. E-mail:kikuma@affrc.go.jp.
Editor: J. L. Flynn
ISSN:0019-9567
1098-5522
DOI:10.1128/IAI.73.6.3778-3782.2005