Estimation of parameters influencing waterborne transmission of infectious hematopoietic necrosis virus (IHNV) in Atlantic salmon (Salmo salar)

• Published in: PloS one Vol. 8; no. 12; p. e82296
• Main Authors: Garver, Kyle A, Mahony, Amelia A M, Stucchi, Dario, Richard, Jon, Van Woensel, Cecile, Foreman, Mike
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 05.12.2013; Public Library of Science (PLoS)
• Subjects: Animals; Aquaculture; Aquatic environment; Biota; Decay rate; Disease control; Disease Transmission, Infectious; Dispersal; Dispersion; Dosage; Environmental management; Fish; Fish Diseases - transmission; Infectious diseases; Infectious hematopoietic necrosis; Infectious hematopoietic necrosis virus; Infectivity; Marine environment; Models, Biological; Mortality; Necrosis; Oncorhynchus mykiss; Parameter estimation; Rhabdoviridae Infections - transmission; Rhabdoviridae Infections - veterinary; Salmo salar; Salmo salar - virology; Salmon; Seawater; Sunlight; Trout; Virus Shedding - physiology; Viruses; Water circulation; Vancouver Island; Canada
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0082296

Understanding how pathogenic organisms spread in the environment is crucial for the management of disease, yet knowledge of propagule dispersal and transmission in aquatic environments is limited. We conducted empirical studies using the aquatic virus, infectious hematopoietic necrosis virus (IHNV), to quantify infectious dose, shedding capacity, and virus destruction rates in order to better understand the transmission of IHN virus among Atlantic salmon marine net-pen aquaculture. Transmission of virus and subsequent mortality in Atlantic salmon post-smolts was initiated with as low as 10 plaque forming units (pfu) ml(-1). Virus shedding from IHNV infected Atlantic salmon was detected before the onset of visible signs of disease with peak shed rates averaging 3.2 × 10(7) pfu fish(-1) hour(-1) one to two days prior to mortality. Once shed into the marine environment, the abundance of free IHNV is modulated by sunlight (UV A and B) and the growth of natural biota present in the seawater. Virus decayed very slowly in sterilized seawater while rates as high as k =  4.37 d(-1) were observed in natural seawater. Decay rates were further accelerated when exposed to sunlight with virus infectivity reduced by six orders of magnitude within 3 hours of full sunlight exposure. Coupling the IHNV transmission parameter estimates determined here with physical water circulation models, will increase the understanding of IHNV dispersal and provide accurate geospatial predictions of risk for IHNV transmission from marine salmon sites.