Characterization of possible correlates of protective response against Brucella ovis infection in rams immunized with the B. melitensis Rev 1 vaccine

• Published in: Vaccine Vol. 27; no. 23; pp. 3039 - 3044
• Main Authors: Galindo, Ruth C, Muñoz, Pilar M, de Miguel, María J, Marin, Clara M, Blasco, José M, Gortazar, Christian, Kocan, Katherine M, de la Fuente, José
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 18.05.2009; Elsevier
• Subjects: Allergy and Immunology; Animals; Annexin A1 - biosynthesis; Annexin A1 - genetics; Applied microbiology; Bacterial diseases; Bacterial Vaccines - therapeutic use; Bacteriology; bcl-2 Homologous Antagonist-Killer Protein - biosynthesis; bcl-2 Homologous Antagonist-Killer Protein - genetics; Biological and medical sciences; Biomarkers - analysis; Biomarkers - metabolism; Brucella; Brucella melitensis; Brucella ovis; Brucella ovis - immunology; Brucellosis - genetics; Brucellosis - immunology; Brucellosis - prevention & control; Brucellosis - veterinary; Fundamental and applied biological sciences. Psychology; Gene Expression; Gene Expression Profiling; Genomics; Human bacterial diseases; Infectious diseases; Male; Medical sciences; Microarray; Microbiology; Miscellaneous; Ovis; Reverse Transcriptase Polymerase Chain Reaction; RNA - analysis; Sheep; Sheep Diseases - genetics; Sheep Diseases - immunology; Sheep Diseases - prevention & control; Toll-Like Receptor 10 - biosynthesis; Toll-Like Receptor 10 - genetics; Vaccine; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects); Vaccines, Attenuated - therapeutic use; Sheep; Vaccine; Microarray; Genomics; Brucella; Immunization; Farming animal; Taxonomy; Infection; Taxonomic review; Vertebrata; Mammalia; Brucellaceae; Bacteriosis; Bacteria; Brucellosis; Artiodactyla; Brucella ovis; Veterinary; Ungulata
• ISSN: 0264-410X; 1873-2518
• DOI: 10.1016/j.vaccine.2009.03.046

Abstract Vaccination with the live attenuated Brucella melitensis Rev 1 vaccine is used to control ovine brucellosis caused by Brucella ovis in sheep. The objective of this study was to identify possible correlates of protective response to B. ovis infection through the characterization by microarray hybridization and real-time RT-PCR of inflammatory and immune response genes differentially expressed in rams previously immunized with B. melitensis Rev 1 and experimentally challenged with B. ovis . Gene expression profiles were compared before and after challenge with B. ovis between rams protected and those vaccinated but found infected after challenge. The TLR10, Bak and ANXI genes were expressed at higher levels in vaccinated and protected rams. These genes provide possible correlates of protective response to B. ovis infection in rams immunized with the B. melitensis Rev 1 vaccine.