Mechanical Properties and Nanomotion of BT-20 and ZR-75 Breast Cancer Cells Studied by Atomic Force Microscopy and Optical Nanomotion Detection Method

Cells of two molecular genetic types of breast cancer—hormone-dependent breast cancer (ZR-75 cell line) and triple-negative breast cancer (BT-20 cell line)—were studied using atomic force microscopy and an optical nanomotion detection method. Using the Peak Force QNM and Force Volume AFM modes, we r...

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Published inCells (Basel, Switzerland) Vol. 12; no. 19; p. 2362
Main Authors Starodubtseva, Maria N, Shkliarava, Nastassia M, Chelnokova, Irina A, Villalba, María I, Krylov, Andrei Yu, Nadyrov, Eldar A, Kasas, Sandor
Format Journal Article
LanguageEnglish
Published Basel MDPI AG 26.09.2023
MDPI
Subjects
Actin
actin cytoskeleton
Actin-depolymerizing protein
Adhesives
Atomic force microscopy
Breast cancer
BT-20 cell
cancer cell
Cancer cells
Cell culture
Cell membranes
Cytochalasin D
Cytoskeleton
Diagnosis
elastic modulus
Estrogens
Genetic aspects
Health aspects
Mechanical properties
Microscopy
nanomotion
Penicillin
Tumor cell lines
viscoelasticity
Belarus
St Louis Missouri
United States > US
Germany
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Summary:Cells of two molecular genetic types of breast cancer—hormone-dependent breast cancer (ZR-75 cell line) and triple-negative breast cancer (BT-20 cell line)—were studied using atomic force microscopy and an optical nanomotion detection method. Using the Peak Force QNM and Force Volume AFM modes, we revealed the unique patterns of the dependence of Young’s modulus on the indentation depth for two cancer cell lines that correlate with the features of the spatial organization of the actin cytoskeleton. Within a 200–300 nm layer just under the cell membrane, BT-20 cells are stiffer than ZR-75 cells, whereas in deeper cell regions, Young’s modulus of ZR-75 cells exceeds that of BT-20 cells. Two cancer cell lines also displayed a difference in cell nanomotion dynamics upon exposure to cytochalasin D, a potent actin polymerization inhibitor. The drug strongly modified the nanomotion pattern of BT-20 cells, whereas it had almost no effect on the ZR-75 cells. We are confident that nanomotion monitoring and measurement of the stiffness of cancer cells at various indentation depths deserve further studies to obtain effective predictive parameters for use in clinical practice.
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ISSN:2073-4409
2073-4409
DOI:10.3390/cells12192362