Effects of heat shock on the Mre11 Rad50 Nbs1 complex in irradiated or unirradiated cells
The mechanism by which hyperthermia sensitizes mammalian cells to ionizing radiation remains to be elucidated, but an overwhelming amount of circumstantial evidence suggests that heat radiosensitization might be mediated by inhibition of double-strand break repair, particularly after exposure of irr...
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Published in | International journal of hyperthermia Vol. 20; no. 2; pp. 144 - 156 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
England
Informa UK Ltd
01.03.2004
Taylor & Francis |
Subjects | |
Online Access | Get full text |
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Summary: | The mechanism by which hyperthermia sensitizes mammalian cells to ionizing radiation remains to be elucidated, but an overwhelming amount of circumstantial evidence suggests that heat radiosensitization might be mediated by inhibition of double-strand break repair, particularly after exposure of irradiated cells to heat treatments in excess of about 43°C. In mammalian cells, double-strand break repair usually occurs via two pathways, non-homologous end-joining and homologous recombination. Several reports suggest a role for non-homologous end-joining in heat radiosensitization, while others implicate homologous recombination as a target. However, cell lines that are compromised in either the non-homologous end-joining or homologous recombination pathway are still capable of being radiosensitized, suggesting that heat affects both pathways. Indeed, several of the proteins involved in one or both of these pathways have been observed to undergo alterations or translocation after unirradiated or irradiated cells are exposed to heat shock. The work summarized in this review implicates proteins of the Mre11 Rad50 Nbs1 complex as targets for heat radiosensitization. |
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Bibliography: | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-3 content type line 23 ObjectType-Review-1 |
ISSN: | 0265-6736 1464-5157 |
DOI: | 10.1080/02656730410001660634 |