Δ-6 Desaturase substrate competition: dietary linoleic acid (18:2n-6) has only trivial effects on α-linolenic acid (18:3n-3) bioconversion in the teleost rainbow trout

• Published in: PloS one Vol. 8; no. 2; p. e57463
• Main Authors: Emery, James A, Hermon, Karen, Hamid, Noor K A, Donald, John A, Turchini, Giovanni M
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 27.02.2013; Public Library of Science (PLoS)
• Subjects: Agriculture; alpha-Linolenic Acid - pharmacology; Animal models; Animals; Aquaculture; Bioconversion; Biology; Competition; Desaturase; Diet; Fatty acids; Feeding Behavior - drug effects; Fish oils; Gene Expression Regulation, Enzymologic - drug effects; Linoleic acid; Linoleic Acid - pharmacology; Linolenic acid; Linoleoyl-CoA Desaturase - genetics; Linoleoyl-CoA Desaturase - metabolism; Lipids; Metabolism; Oncorhynchus mykiss; Oncorhynchus mykiss - growth & development; Oncorhynchus mykiss - metabolism; Oxidation; Polyunsaturated fatty acids; Rodents; Substrate Specificity - drug effects; Substrates; Trout; Vertebrates; Veterinary Science
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0057463

It is generally accepted that, in vertebrates, omega-3 (n-3) and omega-6 (n-6) poly-unsaturated fatty acids (PUFA) compete for Δ-6 desaturase enzyme in order to be bioconverted into long-chain PUFA (LC-PUFA). However, recent studies into teleost fatty acid metabolism suggest that these metabolic processes may not conform entirely to what has been previously observed in mammals and other animal models. Recent work on rainbow trout has led us to question specifically if linoleic acid (LA, 18∶2n-6) and α-linolenic acid (ALA, 18∶3n-3) (Δ-6 desaturase substrates) are in direct competition for access to Δ-6 desaturase. Two experimental diets were formulated with fixed levels of ALA, while LA levels were varied (high and low) to examine if increased availability of LA would result in decreased bioconversion of ALA to its LC-PUFA products through substrate competition. No significant difference in ALA metabolism towards n-3 LC-PUFA was exhibited between diets while significant differences were observed in LA metabolism towards n-6 LC-PUFA. These results are evidence for minor if any competition between substrates for Δ-6 desaturase, suggesting that, paradoxically, the activity of Δ-6 desaturase on n-3 and n-6 substrates is independent. These results call for a paradigm shift in the way we approach teleost fatty acid metabolism. The findings are also important with regard to diet formulation in the aquaculture industry as they indicate that there should be no concern for possible substrate competition between 18∶3n-3 and 18∶2n-6, when aiming at increased n-3 LC-PUFA bioconversion in vivo.